AADAT
AADAT (aminoadipate aminotransferase, also known as KATII, KAT2 and KYAT2) is a mitochondrial transaminase with broad substrate specificity. It acts on aminoadipate and kynurenine, linking lysine catabolism with kynurenine metabolism. Browse Assay Genie's current range of AADAT research reagents below, or read on for the biology behind the target.
AADAT is a transaminase with broad substrate specificity. It shows transaminase activity towards aminoadipate, kynurenine, methionine and glutamate, and also acts on tryptophan, aspartate and hydroxykynurenine. It accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. In vitro, it can also use glyoxylate as an amino-group acceptor.
The AADAT gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II; the mouse and rat orthologs are both named Aadat. The rat protein is a homodimer with two transaminase activities. One is the transamination of alpha-aminoadipic acid, a final step in the saccharopine pathway, the major pathway for L-lysine catabolism. The other is the transamination of kynurenine to produce kynurenic acid, the precursor of kynurenic acid, which has neuroprotective properties. The protein localises to the mitochondrion and belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. HGNC places it in the transaminases gene group.
The names KATII, KAT2 and KYAT2 reflect its role as a kynurenine aminotransferase, while the approved symbol refers to its aminoadipate activity. Several transcript variants encoding two different isoforms have been reported for the AADAT gene. Researchers studying lysine degradation, kynurenine metabolism or mitochondrial amino acid handling often examine AADAT expression and activity alongside related pathway enzymes.
AADAT catalyses transamination of alpha-aminoadipic acid, a final step in the saccharopine pathway, the major route of L-lysine breakdown.
The enzyme transaminates kynurenine to produce kynurenic acid precursor, placing AADAT within kynurenine metabolism.
Kynurenic acid, formed downstream of AADAT activity, has neuroprotective properties, making the enzyme relevant to neurobiology.
With activity towards methionine, glutamate, tryptophan and aspartate, AADAT is a useful model of broad-specificity aminotransferase function.
AADAT is a mitochondrial transaminase with broad substrate specificity. It transaminates aminoadipate, a late step in the saccharopine pathway of L-lysine catabolism, and kynurenine, leading to kynurenic acid. It also acts on methionine, glutamate, tryptophan and aspartate.
AADAT is also called KATII, KAT2 and KYAT2, and UniProt names it kynurenine/alpha-aminoadipate aminotransferase, mitochondrial. NCBI Gene reports several transcript variants encoding two different isoforms. Check the datasheet of any product for its validated reactivity.
Yes. The human protein is highly similar to mouse and rat kynurenine aminotransferase II, and both rodent orthologs are named Aadat. The rat protein has been characterised as a homodimer with two transaminase activities.
Other transaminases, kynurenine pathway enzymes and lysine catabolism proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8N5Z0; product specifications from Assay Genie datasheets.