DOK1
DOK1 (docking protein 1, also known as p62dok) is an enzymatically inert adaptor protein that acts downstream of receptor tyrosine kinases. It provides a docking platform for multimolecular signalling complexes and appears to regulate insulin signalling negatively. Browse Assay Genie's current range of DOK1 research reagents below, or read on for the biology behind the target.
DOK1 is a member of the DOK family of adaptor proteins. Like other DOK proteins, it is enzymatically inert: rather than catalysing a reaction, it acts as a scaffold that provides a docking platform for the assembly of multimolecular signalling complexes. NCBI Gene describes the encoded protein as part of a signal transduction pathway downstream of receptor tyrosine kinases, where it helps form a platform for multiprotein assemblies.
Two specific functions are annotated for DOK1. It appears to be a negative regulator of the insulin signalling pathway, and it modulates integrin activation by competing with talin for the same binding site on ITGB3. These roles illustrate how a scaffold protein can shape signalling output without any catalytic activity of its own. In cells, the DOK1 protein is found in the cytoplasm, the nucleus and the perinuclear region.
The DOK1 gene is classed by HGNC in the docking protein family, and UniProt places the protein in the Type A subfamily of the DOK family. Its approved name, docking protein 1, replaced the earlier form docking protein 1, 62kD (downstream of tyrosine kinase 1), and the alias p62dok is still widely used in the literature. Several transcript variants encoding different isoforms have been reported. Mouse and rat both carry a Dok1 ortholog, which supports comparative work in standard rodent models. Related family members such as DOK2, DOK3, DOK6 and DOK7 are useful for studying how the DOK adaptors differ from one another.
DOK1 sits downstream of receptor tyrosine kinases and assembles multiprotein signalling complexes, making it a useful node for studying signal transduction.
DOK1 appears to act as a negative regulator of the insulin signalling pathway, so it is relevant to studies of how this pathway is restrained.
DOK1 modulates integrin activation by competing with talin for the same binding site on ITGB3, linking adaptor biology to integrin regulation.
As an enzymatically inert DOK family scaffold, DOK1 is a model for how docking platforms organise signalling complexes without catalytic activity.
DOK1 is an enzymatically inert adaptor that provides a docking platform for assembling multimolecular signalling complexes downstream of receptor tyrosine kinases. It appears to negatively regulate insulin signalling and modulates integrin activation by competing with talin for binding to ITGB3.
DOK1 is the approved symbol for docking protein 1, and p62dok is a recognised alias. Earlier HGNC names included docking protein 1, 62kD (downstream of tyrosine kinase 1). NCBI Gene notes that several transcript variants encode different isoforms.
Mouse and rat each have a Dok1 ortholog. Within humans, DOK1 belongs to the DOK family, Type A subfamily, alongside other docking proteins such as DOK2, DOK3, DOK6 and DOK7. Check the datasheet of any product for its validated reactivity.
Other members of the DOK docking protein family with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q99704; product specifications from Assay Genie datasheets.