ACADS
ACADS (acyl-CoA dehydrogenase short chain, also known as SCAD and ACAD3) is a mitochondrial flavoprotein that catalyses the first step of fatty acid beta-oxidation. It acts specifically on short-chain saturated acyl-CoAs. Browse Assay Genie's current range of ACADS research reagents below, or read on for the biology behind the target.
ACADS is one of the acyl-CoA dehydrogenases that catalyse the first step of mitochondrial fatty acid beta-oxidation, an aerobic process that breaks fatty acids down into acetyl-CoA and allows energy to be produced from fats. This first step removes one hydrogen from C-2 and C-3 of a straight-chain fatty acyl-CoA thioester, forming trans-2-enoyl-CoA. Among the mitochondrial acyl-CoA dehydrogenases, ACADS acts specifically on acyl-CoAs with saturated primary chains of 4 to 6 carbons, which is why it is called the short-chain specific enzyme.
The ACADS gene encodes a tetrameric mitochondrial flavoprotein that belongs to the acyl-CoA dehydrogenase family, which HGNC groups with the flavoproteins. The mature enzyme is found in the mitochondrial matrix. Alternative splicing of the gene results in two variants that encode different isoforms. The mouse and rat orthologs are both named Acads.
The protein is listed by UniProt as short-chain specific acyl-CoA dehydrogenase, mitochondrial, and the approved gene name was previously acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain. Mutations in the ACADS gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency, making the enzyme a reference point for studies of inherited disorders of fatty acid oxidation.
ACADS catalyses the initial dehydrogenation step of mitochondrial beta-oxidation for short-chain acyl-CoAs, making it a key enzyme for studying how fats are broken down for energy.
As a matrix flavoprotein of the acyl-CoA dehydrogenase family, ACADS is relevant to work on mitochondrial enzyme function and aerobic energy production from fats.
Mutations in the ACADS gene are associated with short-chain acyl-CoA dehydrogenase deficiency, so the protein is central to studies of this metabolic disorder.
ACADS acts on saturated acyl-CoAs of 4 to 6 carbons, offering a model for comparing chain-length specificity across the mitochondrial acyl-CoA dehydrogenases.
ACADS catalyses the first step of mitochondrial fatty acid beta-oxidation, removing hydrogen from C-2 and C-3 of a fatty acyl-CoA thioester to form trans-2-enoyl-CoA. It acts specifically on saturated acyl-CoAs with primary chains of 4 to 6 carbons.
ACADS is also known as SCAD and ACAD3, and its UniProt name is short-chain specific acyl-CoA dehydrogenase, mitochondrial. The earlier gene name was acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain. Alternative splicing gives two variants encoding different isoforms.
Mutations in the ACADS gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Orthologs exist in mouse and rat, both named Acads; check the datasheet of any product for its validated reactivity.
Other acyl-CoA dehydrogenases and fatty acid beta-oxidation enzymes with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P16219; product specifications from Assay Genie datasheets.