PLS3
PLS3 (plastin 3, also known as T-plastin) is a cytoplasmic actin-bundling protein of the plastin family. It is the T isoform of the plastins, found in solid tissue cells that have replicative potential. Browse Assay Genie's current range of PLS3 research reagents below, or read on for the biology behind the target.
PLS3 is an actin-bundling protein. Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. The PLS3 protein is located in the cytoplasm, where it acts on the actin cytoskeleton. It contains a potential calcium-binding site near the N terminus, consistent with its classification in the HGNC gene group of EF-hand domain containing proteins.
In humans, two ubiquitous plastin isoforms, L and T, have been identified. The L isoform is expressed only in haemopoietic cell lineages, while the T isoform, encoded by the PLS3 gene, has been found in all other normal cells of solid tissues that have replicative potential, including fibroblasts, endothelial cells, epithelial cells and melanocytes. A third distinct isoform, plastin 1 (also known as fimbrin), is expressed at high levels specifically in the small intestine. The C-terminal 570 amino acids of T-plastin and L-plastin are 83% identical. Alternate splicing of the PLS3 gene results in multiple transcript variants.
The approved HGNC name is plastin 3, and the previous name was plastin 3 (T isoform), reflecting the T-plastin alias still used in the literature. Mouse and rat each have an orthologous Pls3 gene. These features make PLS3 a useful reference point for studies of actin organisation in non-haematopoietic cells.
PLS3 is an actin-bundling protein, so it is relevant to studies of how actin filaments are organised in the cytoplasm.
The T, L and fimbrin plastin isoforms differ in tissue distribution, making PLS3 useful for comparing isoform-specific expression.
T-plastin occurs in fibroblasts, endothelial cells, epithelial cells and melanocytes, supporting work on cells of solid tissues with replicative potential.
A potential calcium-binding site near the N terminus places PLS3 among EF-hand domain containing proteins of interest in calcium-regulated function.
PLS3 is an actin-bundling protein of the plastin family, located in the cytoplasm. Plastins are actin-binding proteins conserved across eukaryotes. PLS3 is the T isoform, found in normal solid tissue cells with replicative potential such as fibroblasts, endothelial cells, epithelial cells and melanocytes.
Two ubiquitous human plastin isoforms, L and T, have been described. The L isoform is restricted to haemopoietic lineages, while the T isoform is found in other solid tissue cells. The HGNC previous name was plastin 3 (T isoform). Alternate splicing gives multiple transcript variants.
The C-terminal 570 amino acids of T-plastin and L-plastin are 83% identical, and plastins are conserved throughout eukaryote evolution. Mouse and rat both carry an orthologous Pls3 gene. Check the datasheet of any product for its validated reactivity.
Other plastin family members and similarly named targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P13797; product specifications from Assay Genie datasheets.