LAMC2
LAMC2 (laminin subunit gamma 2) is the gamma 2 chain of the laminin family of extracellular matrix glycoproteins, found in basement membranes. It is also known as nicein-100kDa, kalinin-105kDa, BM600-100kDa, EBR2, LAMB2T, LAMNB2 and EBR2A. Browse Assay Genie's current range of LAMC2 research reagents below, or read on for the biology behind the target.
Laminins are extracellular matrix glycoproteins and the major noncollagenous constituent of basement membranes. Each laminin is a heterotrimer of one alpha, one beta and one gamma chain, arranged in a cruciform structure with three short arms and a long arm made up of all three chains. LAMC2 encodes the gamma 2 chain. Laminin is thought to mediate the attachment, migration and organisation of cells into tissues during embryonic development, through binding to cells via a high affinity receptor and interacting with other extracellular matrix components. The same protein, under the name ladsin, shows cell-scattering activity towards epithelial, endothelial and fibroblastic cells.
The gamma 2 chain is highly homologous to the gamma 1 chain (LAMC1), but it lacks domain VI, and its domains V, IV and III are shorter. It is expressed in several foetal tissues, differently from gamma 1, and is specifically localised to epithelial cells in skin, lung and kidney. Together with the alpha 3 and beta 3 chains it forms laminin 5, earlier known as kalinin, an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. This epithelium-specific expression points to a role for the LAMC2 gene product as an epithelial attachment molecule. Two transcript variants arising from alternative splicing of the 3' terminal exon encode different isoforms and are differentially expressed in embryonic tissues, although their biological significance is not known.
The gene was formerly named for Herlitz junctional epidermolysis bullosa, and mutations in LAMC2 have been associated with junctional epidermolysis bullosa, a skin disease characterised by blisters caused by disruption of the epidermal-dermal junction. Laminins more broadly have been implicated in cell adhesion, differentiation, migration, signalling, neurite outgrowth and metastasis. The mouse ortholog is Lamc2.
LAMC2 is a component of laminin 5 in anchoring filaments, making it a useful subject for studying how epithelial cells attach to the basement membrane.
Mutations in LAMC2 are associated with junctional epidermolysis bullosa, a blistering disease caused by disruption of the epidermal-dermal junction.
Laminin mediates the attachment, migration and organisation of cells, and ladsin activity scatters epithelial, endothelial and fibroblastic cells.
The gamma 2 chain is expressed in several foetal tissues, and its two splice variants are differentially expressed in embryonic tissues.
LAMC2 encodes the laminin gamma 2 chain, which joins the alpha 3 and beta 3 chains to form laminin 5. This laminin is part of the anchoring filaments that connect epithelial cells to the basement membrane, supporting cell attachment, migration and tissue organisation.
LAMC2 is also called nicein-100kDa, kalinin-105kDa, BM600-100kDa, EBR2, LAMB2T, LAMNB2 and EBR2A. The chain was once thought to be a truncated beta chain (B2t), and the protein has also been described as ladsin. Two transcript variants encode different isoforms.
Mutations in LAMC2 have been associated with junctional epidermolysis bullosa, a skin disease marked by blistering at the epidermal-dermal junction. The mouse ortholog is Lamc2. Check the datasheet of any product for its validated reactivity.
Other laminin gamma chains and related basement membrane targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q13753; product specifications from Assay Genie datasheets.