SSRP1
SSRP1 (structure specific recognition protein 1, also known as FACT80) is a subunit of the FACT complex, a general chromatin factor that reorganises nucleosomes. It acts as a histone chaperone during processes that use DNA as a template, including transcription, replication and repair. Browse Assay Genie's current range of SSRP1 research reagents below, or read on for the biology behind the target.
SSRP1 is a component of the FACT complex, a general chromatin factor that reorganises nucleosomes. FACT is involved in several processes that require DNA as a template, such as mRNA elongation, DNA replication and DNA repair. During transcription elongation it acts as a histone chaperone that both destabilises and restores nucleosomal structure. It helps RNA polymerase II pass by promoting dissociation of one histone H2A-H2B dimer from the nucleosome, and then promotes re-establishment of the nucleosome once the polymerase has moved on.
The SSRP1 protein forms a heterodimer with SUPT16H, and together the two subunits make up the chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. SSRP1 contains a high mobility group box, which most likely forms the structure recognition element for cisplatin-modified DNA. The protein binds specifically to double-stranded DNA and, at low levels, to DNA modified by the antitumour agent cisplatin. UniProt places it in the nucleus, including the nucleolus, and on chromosomes, and assigns it to the SSRP1 family. The mouse and rat orthologues are both named Ssrp1.
FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin, and SSRP1 may potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. The FACT complex is probably also involved in phosphorylation of Ser-392 of p53/TP53 through its association with CK2 (casein kinase II). SSRP1 also acts as a transcriptional coactivator for p63/TP63. An alternatively spliced transcript variant of the SSRP1 gene has been described, although its full-length nature is not known.
SSRP1 is part of FACT, which reorganises nucleosomes and acts as a histone chaperone that destabilises and restores nucleosomal structure.
With SUPT16H, SSRP1 supports RNA polymerase II passage by promoting H2A-H2B dimer dissociation and subsequent nucleosome re-establishment.
The FACT complex is involved in DNA replication and repair, and SSRP1 may block replication and repair of cisplatin-modified DNA.
SSRP1 recognises cisplatin-modified DNA, may influence p53 Ser-392 phosphorylation through CK2, and coactivates p63.
SSRP1 is a subunit of the FACT complex, which reorganises nucleosomes as a histone chaperone. It supports mRNA elongation, DNA replication and DNA repair, and helps RNA polymerase II pass through chromatin by promoting H2A-H2B dimer dissociation and later nucleosome re-establishment.
The HGNC name is structure specific recognition protein 1, and UniProt lists the protein as FACT complex subunit SSRP1. FACT80 is a recognised alias. FACT stands for the heterodimeric elongation factor formed by SSRP1 and SUPT16H. An alternatively spliced transcript variant has been described, but its full-length nature is not known.
Mouse and rat both have an Ssrp1 orthologue. SSRP1 contains a high mobility group box that most likely recognises cisplatin-modified DNA, and FACT with damaged DNA may be crucial to cisplatin's anticancer mechanism. Check the datasheet of any product for its validated reactivity.
Other FACT complex partners, histone-chaperone-associated factors and p53 family proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q08945; product specifications from Assay Genie datasheets.