ATP5PF
ATP5PF (ATP synthase peripheral stalk subunit F6, also known as CF6, ATP5A, ATP5, ATPM and ATP5J) is a subunit of the Fo domain of mitochondrial ATP synthase, or Complex V. It forms part of the peripheral stalk and is required for F1 and Fo interactions. Browse Assay Genie's current range of ATP5PF research reagents below, or read on for the biology behind the target.
ATP5PF is subunit F6 of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase, or Complex V). This complex produces ATP from ADP in the presence of a proton gradient across the inner membrane, which is generated by the electron transport complexes of the respiratory chain during oxidative phosphorylation. The enzyme has a soluble F1 head domain, which forms the catalytic core, and a membrane-spanning Fo domain, which forms the proton channel. A central stalk rotating inside the F1 region and a stationary peripheral stalk link the two domains. ATP synthesis in F1 is coupled to proton translocation through a rotary mechanism of the central stalk subunits.
ATP5PF is part of the Fo domain and of the peripheral stalk, which acts as a stator. It holds the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. The NCBI Gene summary describes the F6 subunit as required for F1 and Fo interactions. Fo comprises nine subunits (a, b, c, d, e, f, g, F6 and 8), and the F1 complex has five different subunits (alpha, beta, gamma, delta and epsilon) in a 3:3:1:1:1 ratio. In vivo, the complex can only synthesise ATP, although ATP hydrolase activity can be activated artificially in vitro. The ATP5PF protein belongs to the eukaryotic ATPase subunit F6 family and localises to the mitochondrion inner membrane.
The ATP5PF gene is classed by HGNC in the group of mitochondrial complex V: ATP synthase subunits. Earlier names include ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6, which explains the aliases ATP5A, ATP5, ATPM and ATP5J. Alternatively spliced transcript variants encoding different isoforms have been identified, and the gene has one or more pseudogenes. Mouse and rat orthologs are both named Atp5pf.
ATP5PF contributes to the ATP synthase that makes ATP from ADP using the proton gradient generated by the respiratory chain.
As an Fo subunit, it is a component for studying how electrochemical proton gradients across the inner membrane are coupled to ATP synthesis.
The F6 subunit is required for F1 and Fo interactions, making it relevant to the architecture of the peripheral stalk.
The peripheral stalk acts as a stator holding the alpha(3)beta(3) subcomplex static, a key feature of rotary catalysis in Complex V.
ATP5PF is subunit F6 of the Fo domain of mitochondrial ATP synthase. As part of the peripheral stalk, it acts as a stator that holds the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static, and it is required for F1 and Fo interactions.
The HGNC name is ATP synthase peripheral stalk subunit F6. Aliases and previous symbols include CF6, ATP5A, ATP5, ATPM and ATP5J. Previous names describe it as subunit F6 of the mitochondrial F0 complex. Alternatively spliced transcript variants encoding different isoforms have been reported.
Yes. Mouse and rat each have an ortholog named Atp5pf. The protein belongs to the eukaryotic ATPase subunit F6 family. Check the datasheet of any product for its validated reactivity.
Other ATP synthase subunits and ATPase family members with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P18859; product specifications from Assay Genie datasheets.