SMYD3
SMYD3 (SET and MYND domain containing 3) is a histone-lysine methyltransferase that acts in transcriptional activation as part of an RNA polymerase complex. It is also known as KMT3E, ZNFN3A1 and ZMYND1. Browse Assay Genie's current range of SMYD3 research reagents below, or read on for the biology behind the target.
SMYD3 is a histone methyltransferase. It specifically methylates lysine 4 of histone H3, inducing di- and tri-methylation but not monomethylation, and it also methylates lysine 5 of histone H4. It plays an important role in transcriptional activation as a member of an RNA polymerase complex. According to the NCBI Gene summary, it functions in RNA polymerase II complexes through an interaction with a specific RNA helicase. SMYD3 also binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences, linking its chromatin-modifying activity to sequence recognition.
The human SMYD3 gene encodes a protein that belongs to the class V-like SAM-binding methyltransferase superfamily and the histone-lysine methyltransferase family. HGNC places it in the MYND-type zinc finger group, the SET domain containing group and the non-histone lysine methyltransferases. UniProt records its subcellular location as cytoplasm and nucleus. Multiple transcript variants encoding different isoforms have been reported for the gene. The mouse ortholog is Smyd3.
The gene was previously named zinc finger, MYND domain containing 1, and it appears in the literature under the symbols ZMYND1, ZNFN3A1 and KMT3E. Researchers studying the SMYD3 protein often use it as a model for how SET and MYND domain methyltransferases connect histone marks to transcription. Related family members include SMYD1 and SMYD4.
SMYD3 methylates histone H3 lysine 4 and histone H4 lysine 5, making it a useful enzyme for studying histone marks.
SMYD3 acts within an RNA polymerase complex and supports transcriptional activation, so it is relevant to studies of gene expression control.
SMYD3 functions in RNA polymerase II complexes through an interaction with a specific RNA helicase.
SMYD3 binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' motifs, which supports work on how methyltransferases engage specific sequences.
SMYD3 is a histone methyltransferase. It adds di- and tri-methyl marks to lysine 4 of histone H3, but not monomethyl marks, and also methylates lysine 5 of histone H4. It contributes to transcriptional activation as part of an RNA polymerase complex.
SMYD3 is also called KMT3E, ZNFN3A1 and ZMYND1. Its former HGNC name was zinc finger, MYND domain containing 1. NCBI Gene reports multiple transcript variants encoding different isoforms, so check which isoform any reagent targets.
A mouse ortholog, Smyd3, is recorded for the human gene. The source data do not identify a rat ortholog. Check the datasheet of any product for its validated reactivity before planning cross-species experiments.
Other SMYD family methyltransferases with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9H7B4; product specifications from Assay Genie datasheets.