Anti-SMYD3 Antibody (CAB14516)

Antibody Name:	Anti-SMYD3 Antibody
Antibody SKU:	CAB14516
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SMYD3 (NP_001161212.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, HT-29, HepG2, MCF7, HeLa, Mouse testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SMYD3 (NP_001161212.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DRCL LGKE KLMR CSQC RVAK YCSA KCQK KAWP DHKR ECKC LKSC KPRY PPDS VRLL GRVV FKLM DGAP SESE KLYS FYDL ESNI NKLT EDKK EGLR QLVM T
Gene ID:	64754
Uniprot:	Q9H7B4
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	29kDa/42kDa/49kDa
Observed MW:	49kDa

Synonyms:	SMYD3, KMT3E, ZMYND1, ZNFN3A1, bA74P14.1
Background:	This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SMYD3: Histone methyltransferase. Specifically methylates 'Lys- 4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Belongs to the histone-lysine methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase Chromosomal Location of Human Ortholog: 1q44 Cellular Component: nucleoplasm; cytoplasm Molecular Function:metal ion binding; histone-lysine N-methyltransferase activity Biological Process: nucleosome assembly; establishment and/or maintenance of chromatin architecture; establishment of protein localization; myotube cell development; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation
NCBI Summary:	This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q9H7B4
NCBI GenInfo Identifier:	212276523
NCBI Gene ID:	64754
NCBI Accession:	Q9H7B4.4
UniProt Secondary Accession:	Q9H7B4,Q86TL8, Q8N5Z6, Q96AI5, A8K0P0, B1AN38,
UniProt Related Accession:	Q9H7B4
Molecular Weight:	428
NCBI Full Name:	Histone-lysine N-methyltransferase SMYD3
NCBI Synonym Full Names:	SET and MYND domain containing 3
NCBI Official Symbol:	SMYD3
NCBI Official Synonym Symbols:	KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
NCBI Protein Information:	histone-lysine N-methyltransferase SMYD3; bA74P14.1 (novel protein); zinc finger, MYND domain containing 1; SET and MYND domain-containing protein 3; zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing)
UniProt Protein Name:	Histone-lysine N-methyltransferase SMYD3
UniProt Synonym Protein Names:	SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1
Protein Family:	Histone-lysine N-methyltransferase
UniProt Gene Name:	SMYD3
UniProt Entry Name:	SMYD3_HUMAN

	Western blot analysis of extracts of various cell lines, using SMYD3 antibody (CAB14516) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Human colon using SMYD3 Rabbit pAb (CAB14516) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using SMYD3 antibody (CAB14516) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using SMYD3 antibody (CAB14516) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using SMYD3 antibody (CAB14516) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-SMYD3 Antibody (CAB7309)	Human SMYD3 (SET and MYND domain containing 3) ELISA Kit (HUFI08451)
SMYD3 Antibody (PACO12353)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)