Anti-TST Antibody (CAB10542)

Antibody Name:	Anti-TST Antibody
Antibody SKU:	CAB10542
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human TST (NP_003303.2).

Application:	WB IP
Recommended Dilution:	WB 1:1000 - 1:2000 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, DU145, MCF-7, 293T, Mouse liver, Mouse kidney, Mouse lung, Rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human TST (NP_003303.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVHQ VLYR ALVS TKWL AESI RTGK LGPG LRVL DASW YSPG TREA RKEY LERH VPGA SFFD IEEC RDTA SPYE MMLP SEAG FAEY VGRL GISN HTHV VVYD GEHL GSFY APRV WWMF RVFG HRTV SVLN GGFR NWLK EGHP VTSE PSRP EPAV FKAT LDRS LLKT YEQV LENL ESKR FQLV DSRS QGRF LGTE PEPD AVGL DSGH IRGA VNMP FMDF LTED GFEK GPEE LRAL FQTK KVDL SQPL IATC RKGV TACH VALA AYLC GKPD VAVY DGSW SEWF RRAP PESR VSQG KSEK A
Gene ID:	7263
Uniprot:	Q16762
Cellular Location:	Mitochondrion matrix
Calculated MW:	33kDa
Observed MW:	36kDa

Synonyms:

TST, RDS

Background:

This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	TST: Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
UniProt Protein Details:	Protein type:EC 2.8.1.1; Mitochondrial; Transferase Chromosomal Location of Human Ortholog: 22q12.3 Cellular Component: extracellular space; mitochondrion Molecular Function:5S rRNA binding Biological Process: cyanate catabolic process; epithelial cell differentiation; rRNA transport
NCBI Summary:	This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
UniProt Code:	Q16762
NCBI GenInfo Identifier:	3122965
NCBI Gene ID:	7263
NCBI Accession:	Q16762.4
UniProt Secondary Accession:	Q16762,Q6IB06, B3KRM1,
UniProt Related Accession:	Q16762
Molecular Weight:	33kDa
NCBI Full Name:	Thiosulfate sulfurtransferase
NCBI Synonym Full Names:	thiosulfate sulfurtransferase
NCBI Official Symbol:	TST
NCBI Official Synonym Symbols:	RDS
NCBI Protein Information:	thiosulfate sulfurtransferase
UniProt Protein Name:	Thiosulfate sulfurtransferase
UniProt Synonym Protein Names:	Rhodanese
Protein Family:	Toxic shock syndrome toxin
UniProt Gene Name:	TST

	Western blot analysis of extracts of various cell lines, using TST antibody (CAB10542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug TST antibody (CAB10542). Western blot was performed from the immunoprecipitate using TST antibody (CAB10542) at a dilition of 1:1000.

Antibodies

TST Antibody (PACO12968)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)