RFTN1
RFTN1 (raftlin, lipid raft linker 1; also known as MIG2 and KIAA0084) is a membrane raft-associated protein of the raftlin family. It links clathrin- and AP2-dependent trafficking to innate and adaptive immune receptor signalling. Browse Assay Genie's current range of RFTN1 research reagents below, or read on for the biology behind the target.
RFTN1 is involved in protein trafficking through its association with clathrin and the AP2 complex. After stimulation with bacterial lipopolysaccharide, it mediates internalisation of TLR4 to endosomes in dendritic cells and macrophages. It also mediates internalisation of poly(I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells. This activates TICAM1-mediated signalling and subsequent IFNB1 production. The RFTN1 protein is also linked to double-stranded RNA binding, dsRNA transport and the response to exogenous dsRNA.
In the adaptive immune system, RFTN1 contributes to T-cell antigen receptor-mediated signalling by regulating the localisation of the tyrosine kinase LCK. It also affects T-cell dependent antibody production and cytokine secretion. It may regulate B-cell antigen receptor-mediated signalling, and it is annotated as part of the B cell receptor signalling pathway. RFTN1 may play a pivotal role in the formation and maintenance of lipid rafts. Annotated roles include membrane raft assembly and positive regulation of growth rate.
The RFTN1 gene encodes a protein found at the cell membrane, in the cytoplasm, in membrane rafts and in endosomes, including early endosomes. It belongs to the raftlin family and is part of a protein-containing complex. Aliases include MIG2, KIAA0084 and FLJ23866. The mouse ortholog is Rftn1. NCBI Gene lists RFTN1 as a biomarker of glaucoma, making it relevant to studies beyond immune signalling.
RFTN1 mediates TLR4 and TLR3 internalisation to endosomes, leading to TICAM1-mediated signalling and IFNB1 production.
RFTN1 regulates LCK localisation in T-cell receptor signalling and may regulate B-cell antigen receptor signalling.
RFTN1 may be important in the formation and maintenance of lipid rafts, and it is annotated to membrane raft assembly.
RFTN1 associates with clathrin and AP2 in trafficking, and is listed as a biomarker of glaucoma.
RFTN1 is involved in protein trafficking through clathrin and the AP2 complex. It mediates TLR4 and TLR3 internalisation to endosomes, regulates LCK localisation in T-cell receptor signalling, and may help form or maintain lipid rafts.
RFTN1 stands for raftlin, lipid raft linker 1. The protein is called raftlin, and aliases include MIG2, KIAA0084 and FLJ23866. These names all refer to the same gene, which belongs to the raftlin family.
A mouse ortholog, Rftn1, is recorded. NCBI Gene lists RFTN1 as a biomarker of glaucoma. Check the datasheet of any product for its validated reactivity.
Toll-like receptors, signalling adaptors and T-cell kinases that act alongside RFTN1 in immune signalling.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q14699; product specifications from Assay Genie datasheets.