CARF
CARF (calcium responsive transcription factor) is a nuclear transcriptional activator that links calcium signals to gene expression. It is also known as FLJ21579, NYD-SP24 and ALS2CR8, and binds a defined calcium-response element in DNA. Browse Assay Genie's current range of CARF research reagents below, or read on for the biology behind the target.
CARF acts as a transcriptional activator that mediates the calcium- and neuron-selective induction of BDNF exon III transcription. It binds the consensus calcium-response element CaRE1, with the sequence 5'-CTATTTCGAG-3'. According to the NCBI Gene summary, it enables DNA binding and RNA polymerase II-specific DNA-binding transcription activator activity, and it is involved in the cellular response to calcium ion.
The CARF protein is localised to the nucleus. The NCBI Gene summary also predicts that it is located in the granular component, and active in the nucleus. The human CARF gene has orthologues in the mouse, where it is named Carf, and in the rat, where it is listed as Cdkn2aip. This makes rodent models a practical route for studying its function.
The gene has carried several names. Its previous name, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8, gave rise to the symbol ALS2CR8. Other aliases include FLJ21579 and NYD-SP24. The current HGNC name, calcium responsive transcription factor, reflects its characterised role in calcium-dependent transcription. Researchers studying CARF, whether through a CARF antibody, a CARF ELISA kit or recombinant CARF protein, will often be examining how neuronal activity is converted into transcriptional change.
CARF mediates the cellular response to calcium ion through DNA binding and RNA polymerase II-specific transcriptional activation.
CARF drives calcium- and neuron-selective induction of BDNF exon III transcription, making it a focus for studies of activity-dependent expression.
CARF binds the consensus CaRE1 sequence 5'-CTATTTCGAG-3', which supports work on calcium-response elements and DNA-protein interactions.
CARF is a nuclear protein, predicted to be located in the granular component, which is relevant to subnuclear localisation studies.
CARF is a transcriptional activator. It mediates the calcium- and neuron-selective induction of BDNF exon III transcription and binds the calcium-response element CaRE1. Its activities include DNA binding and RNA polymerase II-specific transcription activation, and it contributes to the cellular response to calcium ion.
CARF is the HGNC symbol for calcium responsive transcription factor. Aliases and previous symbols include FLJ21579, NYD-SP24 and ALS2CR8. The ALS2CR8 symbol comes from the earlier name amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8.
Yes. The mouse orthologue is Carf and the rat orthologue is listed as Cdkn2aip. This allows the biology of human CARF to be explored in rodent systems. Check the datasheet of any product for its validated reactivity.
Neurotrophic factors and calcium-signalling targets linked to CARF biology with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8N187; product specifications from Assay Genie datasheets.