CLP1
CLP1 (cleavage factor polyribonucleotide kinase subunit 1, also known as HEAB or hClp1) is a nuclear polynucleotide kinase that phosphorylates the 5'-hydroxyl groups of RNA and DNA substrates. It acts in tRNA splicing and in mRNA 3'-end formation. Browse Assay Genie's current range of CLP1 research reagents below, or read on for the biology behind the target.
CLP1 is a polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA, single-stranded RNA, double-stranded DNA and double-stranded DNA:RNA hybrids. Double-stranded RNA is phosphorylated more efficiently than double-stranded DNA, and in a DNA:RNA hybrid the RNA component is the preferred substrate. The protein is a multifunctional kinase with roles in tRNA, mRNA and siRNA maturation.
In tRNA splicing, CLP1 is a component of the tRNA splicing endonuclease complex. It phosphorylates the 5'-terminus of the tRNA 3'-exon, and this phosphorylation is a prerequisite for ligation of the two exon halves and production of a mature tRNA. This role in tRNA splicing and maturation is required for cerebellar development. CLP1 is also a component of the pre-mRNA cleavage complex II (CF-II), which appears to be required for mRNA 3'-end formation.
CLP1 also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs, a necessary step for their incorporation into the RNA-induced silencing complex (RISC). Endogenous siRNAs and microRNAs produced by DICER1 cleavage already carry a 5'-phosphate, so the protein may be dispensable for normal RNA-mediated gene silencing. The CLP1 gene encodes a member of the Clp1 family, Clp1 subfamily, and alternatively spliced transcript variants encoding different isoforms have been described. Its former HGNC name refers to the cleavage and polyadenylation factor I subunit, homolog of the S. cerevisiae protein. Mutations in the gene are associated with pontocerebellar hypoplasia type 10 (PCH10).
CLP1 phosphorylates the tRNA 3'-exon within the splicing endonuclease complex, a step required before exon ligation yields mature tRNA.
As a component of pre-mRNA cleavage complex II, CLP1 is studied in the cleavage events that define mRNA 3' ends.
CLP1 phosphorylates the 5'-end of exogenous siRNAs, a prerequisite for RISC incorporation, making it relevant to siRNA processing.
CLP1 mutations are associated with pontocerebellar hypoplasia type 10, and its tRNA splicing role is required for cerebellar development.
CLP1 is a polynucleotide 5'-hydroxyl kinase. It phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing, which is needed for ligation into mature tRNA. It is also part of pre-mRNA cleavage complex II, involved in mRNA 3'-end formation, and phosphorylates exogenous siRNAs.
CLP1 is also called HEAB and hClp1, and its protein name is polyribonucleotide 5'-hydroxyl-kinase Clp1. Earlier HGNC names refer to cleavage and polyadenylation factor I subunit 1. NCBI Gene reports alternatively spliced transcript variants encoding different isoforms.
Yes. According to the NCBI Gene summary, mutations in the CLP1 gene are associated with pontocerebellar hypoplasia type 10 (PCH10). UniProt notes that the protein's role in tRNA splicing and maturation is required for cerebellar development.
DICER1, a source of 5'-phosphorylated small RNAs, and other targets with similar symbols that are listed at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q92989; product specifications from Assay Genie datasheets.