Factor X
Factor X, encoded by the RFXANK gene and also known as BLS, RFX-B and ANKRA1, is an ankyrin repeat protein that forms part of a complex activating MHC class II gene transcription. It is central to the control of antigen-presenting gene expression in the immune system. Browse Assay Genie's current range of Factor X research reagents below, or read on for the biology behind the target.
Major histocompatibility complex (MHC) class II molecules are transmembrane proteins with a central role in the development and control of the immune system. Factor X, the protein product of the RFXANK gene, contributes to their expression. Together with regulatory factor X-associated protein and regulatory factor 5, it forms a complex that binds the X box motif found in certain MHC class II gene promoters and activates their transcription.
Once the complex is bound to the promoter, it associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Factor X contains ankyrin repeats, which are involved in protein-protein interactions. UniProt lists the protein as DNA-binding protein RFXANK and records it in both the cytoplasm and the nucleus. The Factor X gene is reported to give rise to multiple alternatively spliced transcript variants encoding different isoforms; isoform 2 is not involved in the positive regulation of MHC class II genes.
The gene belongs to the HGNC group of ankyrin repeat domain containing genes. Its previous name was regulatory factor X-associated ankyrin-containing protein, and further aliases include BLS, RFX-B, ANKRA1, F14150_1 and MGC138628. Mutations in the Factor X gene have been linked to bare lymphocyte syndrome type II, complementation group B, a disorder of MHC class II expression. The mouse ortholog is Rfxank.
Factor X is part of the complex that binds the X box of MHC class II promoters and activates their transcription.
MHC class II molecules have a central role in immune development and control, making their transcriptional regulators relevant to immunology.
Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B.
Its ankyrin repeats mediate protein-protein interactions, including assembly into a promoter-bound complex that recruits the MHC class II transactivator.
Factor X forms a complex with regulatory factor X-associated protein and regulatory factor 5 that binds the X box motif of certain MHC class II promoters and activates transcription. The bound complex then associates with the MHC class II transactivator, which controls cell type specificity and inducibility.
The gene symbol is RFXANK, with aliases BLS, RFX-B, ANKRA1, F14150_1 and MGC138628. UniProt names it DNA-binding protein RFXANK. Multiple splice variants encode different isoforms; isoform 2 is not involved in the positive regulation of MHC class II genes.
Mutations in the gene have been linked to bare lymphocyte syndrome type II, complementation group B. A mouse ortholog, Rfxank, is recorded. Check the datasheet of any product for its validated reactivity.
Other components of the MHC class II transcriptional complex and MHC class II molecules with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O14593; product specifications from Assay Genie datasheets.