COLGALT1
COLGALT1 (collagen beta(1-O)galactosyltransferase 1, also known as GLT25D1 and FLJ22329) is a beta-galactosyltransferase of the endoplasmic reticulum that glycosylates hydroxylysine residues of collagen. In doing so it supports formation of the collagen triple helix. Browse Assay Genie's current range of COLGALT1 research reagents below, or read on for the biology behind the target.
COLGALT1, also called procollagen galactosyltransferase 1, is a beta-galactosyltransferase that transfers beta-galactose to hydroxylysine residues of type I collagen. By acting on collagen glycosylation, it facilitates the formation of the collagen triple helix. It is also involved in the biosynthesis of collagen type IV. The enzyme is classified under EC 2.4.1.50.
The COLGALT1 gene encodes a soluble protein that localises to the lumen of the endoplasmic reticulum. According to the NCBI Gene summary, it is one of two enzymes that transfer galactose moieties to hydroxylysine residues of collagen and of mannose binding lectin, and the gene is constitutively expressed. The other enzyme is COLGALT2. The protein belongs to the glycosyltransferase 25 family, and the HGNC places it in the collagen beta(1-O)galactosyltransferases gene group. A mouse ortholog, Colgalt1, is recorded.
The gene was previously named glycosyltransferase 25 domain containing 1, with the symbol GLT25D1, and FLJ22329 is also listed as an alias. Researchers studying the COLGALT1 gene or protein are typically interested in collagen post-translational modification, since hydroxylysine galactosylation is one step in preparing collagen for correct triple helix assembly.
COLGALT1 galactosylates hydroxylysine residues of type I collagen and contributes to the biosynthesis of collagen type IV.
By acting on collagen glycosylation, the enzyme facilitates formation of the collagen triple helix.
COLGALT1 is a member of the glycosyltransferase 25 family and transfers beta-galactose to hydroxylysine, classified as EC 2.4.1.50.
As a soluble, constitutively expressed enzyme of the endoplasmic reticulum lumen, it is a model for studying glycosylation within the secretory pathway.
COLGALT1 is a beta-galactosyltransferase that transfers beta-galactose to hydroxylysine residues of type I collagen. This glycosylation facilitates formation of the collagen triple helix, and the enzyme is also involved in the biosynthesis of collagen type IV. It acts within the endoplasmic reticulum lumen.
COLGALT1 stands for collagen beta(1-O)galactosyltransferase 1. UniProt names it procollagen galactosyltransferase 1. Earlier names include GLT25D1 (glycosyltransferase 25 domain containing 1) and the alias FLJ22329. Literature and datasheets may use any of these names for the same gene and protein.
COLGALT1 is one of two enzymes that galactosylate hydroxylysine residues of collagen and mannose binding lectin; the other is COLGALT2. A mouse ortholog, Colgalt1, is recorded. Check the datasheet of any product for its validated reactivity.
COLGALT2, the second collagen hydroxylysine galactosyltransferase, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8NBJ5; product specifications from Assay Genie datasheets.