MFF
MFF (mitochondrial fission factor, also known as GL004 and formerly C2orf33) is a protein that plays a role in mitochondrial and peroxisomal fission. It recruits the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. Browse Assay Genie's current range of MFF research reagents below, or read on for the biology behind the target.
MFF functions in the division of two organelles, mitochondria and peroxisomes. Its best-defined role is to promote the recruitment and association of dynamin-related protein 1 (DNM1L), the fission mediator, with the mitochondrial surface. By bringing DNM1L to the organelle, MFF contributes to the machinery that divides mitochondria. UniProt also notes that MFF may help regulate synaptic vesicle membrane dynamics by recruiting DNM1L to clathrin-containing vesicles.
The MFF protein belongs to the Tango11 family. It is annotated at the mitochondrion outer membrane and at the peroxisome, and it is also found in the cytoplasmic vesicle compartment, specifically synaptic vesicles. This distribution fits its role in organelle fission and in membrane dynamics at the synapse.
The MFF gene is a nuclear gene. According to the NCBI Gene summary, there are multiple pseudogenes on chromosomes 1, 5 and X, and alternative splicing produces multiple transcript variants. Researchers should keep this in mind when designing nucleic acid assays. The gene was previously designated chromosome 2 open reading frame 33 (C2orf33) and is also known by the alias GL004. Mouse and rat both have an orthologous Mff gene, which allows the fission pathway to be studied in common model systems.
MFF recruits DNM1L to the mitochondrial surface, making it a key protein for studying how mitochondrial fission is organised.
MFF plays a role in peroxisomal as well as mitochondrial fission, so it is relevant to research on peroxisome division.
MFF may regulate synaptic vesicle membrane dynamics by recruiting DNM1L to clathrin-containing vesicles.
Its location on the outer mitochondrial membrane, peroxisomes and vesicles makes MFF useful for studying how fission machinery is targeted to membranes.
MFF plays a role in mitochondrial and peroxisomal fission. It promotes the recruitment and association of the fission mediator DNM1L (dynamin-related protein 1) with the mitochondrial surface. It may also help regulate synaptic vesicle membrane dynamics by recruiting DNM1L to clathrin-containing vesicles.
MFF is also called GL004, and its previous symbol was C2orf33, from the name chromosome 2 open reading frame 33. The NCBI Gene summary reports that alternative splicing results in multiple transcript variants, and that pseudogenes exist on chromosomes 1, 5 and X.
Yes. Mouse and rat each have an orthologous gene named Mff, so the protein can be studied in common rodent models. The human protein belongs to the Tango11 family. Check the datasheet of any product for its validated reactivity.
DNM1L and other mitochondrial fission and organelle dynamics proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9GZY8; product specifications from Assay Genie datasheets.