POLR2C
POLR2C (RNA polymerase II subunit C, also known as RPB3) is a core component of RNA polymerase II, the DNA-dependent RNA polymerase that synthesises mRNA precursors and many functional non-coding RNAs. It is the third largest subunit of the enzyme and is found in the nucleus. Browse Assay Genie's current range of POLR2C research reagents below, or read on for the biology behind the target.
POLR2C is a core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase. Pol II uses the four ribonucleoside triphosphates as substrates to synthesise mRNA precursors and many functional non-coding RNAs. In eukaryotes it is the polymerase responsible for producing messenger RNA, so its subunits underpin the first step of protein-coding gene expression.
The POLR2C gene encodes the third largest subunit of Pol II, and the protein is localised to the nucleus. The product contains a cysteine-rich region and exists as a heterodimer with another polymerase subunit, POLR2J. Together, these two subunits form a core subassembly unit of the polymerase. The protein belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family, which reflects the shared ancestry of RNA polymerase machinery across domains of life. HGNC places the gene in the RNA polymerase subunits group. A pseudogene has been identified on chromosome 21, which is worth bearing in mind when designing nucleic acid-based assays for the POLR2C gene.
The protein is also known as RPB3, and the gene was previously named polymerase (RNA) II (DNA directed) polypeptide C (33kD). The mouse ortholog is Polr2c. Researchers studying transcription often work with POLR2C alongside other polymerase subunits, including POLR2A, as well as the subunits of RNA polymerases I and III, when they examine how the transcription apparatus is assembled and organised.
POLR2C is a core Pol II subunit, making it relevant to work on how the polymerase that synthesises mRNA precursors is built and functions.
Its heterodimer with POLR2J forms a core subassembly unit of the polymerase, a useful focus for studies of subunit interactions.
Pol II synthesises many functional non-coding RNAs as well as mRNA precursors, so its subunits are relevant to wider RNA output.
As a member of the archaeal Rpo3/eukaryotic RPB3 family, POLR2C offers a route into comparing RNA polymerase subunits across species.
POLR2C is a core component of RNA polymerase II, which synthesises mRNA precursors and many functional non-coding RNAs from ribonucleoside triphosphates. It forms a heterodimer with POLR2J, and the two subunits make up a core subassembly unit of the polymerase within the nucleus.
POLR2C is also called RPB3, and its UniProt protein name is DNA-directed RNA polymerase II subunit RPB3. The gene was previously named polymerase (RNA) II (DNA directed) polypeptide C (33kD). Its full HGNC name is RNA polymerase II subunit C.
POLR2C belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family, so related subunits exist beyond eukaryotes. The mouse ortholog is Polr2c. A pseudogene has also been identified on human chromosome 21. Check the datasheet of any product for its validated reactivity.
Other RNA polymerase I, II and III subunits with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P19387; product specifications from Assay Genie datasheets.