ADAMTS15
ADAMTS15 (ADAM metallopeptidase with thrombospondin type 1 motif 15) is a secreted metalloprotease of the ADAMTS family. It cleaves the proteoglycan versican (VCAN) in the pericellular matrix and is linked to skeletal muscle development and regeneration. Browse Assay Genie's current range of ADAMTS15 research reagents below, or read on for the biology behind the target.
ADAMTS15 is a metalloprotease with proteolytic activity against the proteoglycan versican (VCAN), which it cleaves at the Glu-1428-|-1429-Ala site. It acts on VCAN in the pericellular matrix surrounding myoblasts, where this cleavage facilitates myoblast contact and fusion. That process is required for skeletal muscle development and regeneration. The protein is found in the extracellular space, within the extracellular matrix, and at the cell surface.
The ADAMTS15 gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of this family share several modules: a propeptide region, a metalloproteinase domain, a disintegrin-like domain and a thrombospondin type 1 (TS) motif. Individual members differ in the number of C-terminal TS motifs, and some carry unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme, which may play a role in versican processing during skeletal muscle development. The mouse ortholog is Adamts15.
HGNC places the gene in the group of ADAM metallopeptidases with thrombospondin type 1 motif. An earlier name was a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 15. According to the NCBI Gene summary, the ADAMTS15 gene may also function as a tumour suppressor in colorectal and breast cancers, which makes the protein of interest beyond muscle biology and matrix remodelling.
ADAMTS15 may contribute to versican processing during skeletal muscle development, and its activity supports muscle regeneration.
Cleavage of VCAN in the pericellular matrix around myoblasts facilitates myoblast contact and fusion.
As a secreted metalloprotease acting on the proteoglycan VCAN, ADAMTS15 is relevant to proteolytic turnover of the extracellular matrix.
The gene may function as a tumour suppressor in colorectal and breast cancers, motivating expression and functional studies.
ADAMTS15 is a metalloprotease that cleaves the proteoglycan versican (VCAN) at the Glu-1428-|-1429-Ala site. It does so in the pericellular matrix around myoblasts, facilitating myoblast contact and fusion, which is required for skeletal muscle development and regeneration.
ADAMTS15 belongs to the ADAMTS family, whose members share a propeptide region, a metalloproteinase domain, a disintegrin-like domain and a thrombospondin type 1 motif. Members differ in the number of C-terminal TS motifs. The preproprotein is processed to give the mature enzyme.
The NCBI Gene summary states that ADAMTS15 may function as a tumour suppressor in colorectal and breast cancers. A mouse ortholog, Adamts15, exists; check the datasheet of any product for its validated reactivity.
Other ADAMTS family metalloproteases involved in extracellular matrix and proteoglycan processing.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8TE58; product specifications from Assay Genie datasheets.