RPS27A
RPS27A (ribosomal protein S27a) encodes a fusion protein in which ubiquitin sits at the N terminus and the 40S ribosomal protein S27a at the C terminus. It is also known as UBCEP80, Uba80, S27A and eS31. Browse Assay Genie's current range of RPS27A research reagents below, or read on for the biology behind the target.
RPS27A is a component of the 40S subunit of the ribosome. It is part of the small subunit (SSU) processome, the first precursor of the small eukaryotic ribosomal subunit. During SSU processome assembly in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA. Together they generate RNA folding, modifications, rearrangements and cleavage, as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
The RPS27A gene encodes a fusion protein: ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. Ubiquitin is a highly conserved protein with a major role in targeting cellular proteins for degradation by the 26S proteasome, and it is synthesised as a precursor consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. When the RPS27A protein is expressed in yeast, it is processed after translation into free ubiquitin monomer and ribosomal protein S27a. The S27a portion belongs to the eukaryotic ribosomal protein eS31 family (S27AE family) and contains C4-type zinc finger domains. UniProt records it in the cytoplasm, nucleolus and nucleus.
RPS27A is one of several ribosomal proteins made as ubiquitin-type fusions. Ribosomal protein L40 is also synthesised as a fusion with ubiquitin, and ribosomal protein S30 as a fusion with the ubiquitin-like protein fubi. HGNC places the gene in both the S ribosomal protein and ubiquitin groups. Pseudogenes derived from the gene are present in the genome, and several alternatively spliced transcript variants encode the same protein. Mouse and rat each have an orthologous Rps27a gene.
RPS27A is part of the SSU processome, which assembles on nascent pre-rRNA in the nucleolus to form the precursor of the small ribosomal subunit.
As a component of the 40S ribosomal subunit, RPS27A is studied alongside other small-subunit proteins in work on ribosome structure and composition.
The ubiquitin-S27a fusion is cleaved to give free ubiquitin and ribosomal protein S27a, making it a model for ubiquitin-fusion precursors.
SSU processome assembly involves RNA folding, cleavage and exosome-mediated degradation of pre-ribosomal RNA, processes in which RPS27A-containing complexes take part.
RPS27A is a component of the 40S ribosomal subunit and part of the small subunit processome, the first precursor of the small subunit, which assembles in the nucleolus. The gene also encodes an N-terminal ubiquitin that is released by post-translational processing.
RPS27A is also called UBCEP80, Uba80, S27A and eS31, and UniProt names it ubiquitin-ribosomal protein eS31 fusion protein. The RPS27A gene encodes one polypeptide with ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. Several splice variants encode the same protein.
Yes. Mouse and rat each have an orthologous Rps27a gene, and the fusion has been processed into free ubiquitin and S27a when expressed in yeast. Ubiquitin itself is highly conserved. Check the datasheet of any product for its validated reactivity.
Other 40S ribosomal proteins that, like RPS27A, are components of the small ribosomal subunit.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P62979; product specifications from Assay Genie datasheets.