ABCC5
ABCC5 (ATP binding cassette subfamily C member 5, also known as MRP5, SMRP, EST277145 and MOAT-C) is an ATP-dependent transporter of the ABC family that extrudes physiological compounds and xenobiotics from cells. It exports cyclic nucleotides and contributes to drug resistance. Browse Assay Genie's current range of ABCC5 research reagents below, or read on for the biology behind the target.
ABCC5 is an ATP-dependent transporter that actively extrudes physiological compounds and xenobiotics from cells. It mediates ATP-dependent transport of endogenous metabolites such as cAMP and cGMP, folic acid and N-lactoyl-amino acids (shown in vitro). It also acts as a general glutamate conjugate and analogue transporter, and in this role can limit the brain levels of endogenous metabolites, drugs and toxins. ABCC5 also acts as a heme transporter, required for translocation of cytosolic heme to the secretory pathway, and may influence energy metabolism by regulating glucagon-like peptide 1 (GLP-1) secretion from enteroendocrine cells.
The ABCC5 gene encodes a member of the ABC transporter superfamily, which move various molecules across extra- and intracellular membranes. The protein belongs to the ABCC family and the conjugate transporter subfamily, and is part of the MRP subfamily associated with multidrug resistance. UniProt records its localisation to the basolateral and apical cell membrane, the Golgi apparatus lumen, endosome membrane and cytoplasmic granules. Alternative splicing produces multiple transcript variants. The mouse and rat orthologues are both named Abcc5.
Export of cyclic nucleotides by ABCC5 contributes to the degradation of phosphodiesterases and possibly provides an elimination pathway for cyclic nucleotides. The protein confers resistance to the antiviral agent PMEA (9-(2-phosphonylmethoxyethyl)adenine) and to the thiopurine anticancer drugs 6-mercaptopurine and thioguanine. It may therefore be involved in thiopurine resistance in acute lymphoblastic leukaemia and in resistance to antiretroviral nucleoside analogues in HIV-infected patients. It can transport methotrexate and other nucleotide analogues in vitro, but with low affinity, so its exact role in resistance remains to be established. The earlier name was ATP-binding cassette, sub-family C (CFTR/MRP), member 5.
ABCC5 belongs to the MRP subfamily and exports thiopurines and nucleoside analogues, making it relevant to studies of drug efflux and resistance.
ABCC5 exports cAMP and cGMP, linking transporter activity to phosphodiesterase degradation and the elimination of cyclic nucleotides.
ABCC5 translocates cytosolic heme to the secretory pathway, so it is studied in intracellular heme transport.
ABCC5 may regulate GLP-1 secretion and limit brain levels of metabolites, drugs and toxins as a glutamate conjugate transporter.
ABCC5 is an ATP-dependent efflux transporter. It exports cAMP, cGMP, folic acid and glutamate conjugates, and moves cytosolic heme into the secretory pathway. It also exports xenobiotics, including antiviral and thiopurine drugs, which is why it is linked to drug resistance.
ABCC5 is also called MRP5 (multidrug resistance-associated protein 5), SMRP, EST277145 and MOAT-C. Its earlier HGNC name was ATP-binding cassette, sub-family C (CFTR/MRP), member 5. Alternative splicing gives multiple transcript variants. Check the datasheet of any product for its validated reactivity.
Mouse and rat both have an orthologue named Abcc5. In humans, ABCC5 may contribute to thiopurine resistance in acute lymphoblastic leukaemia and to resistance to antiretroviral nucleoside analogues in HIV-infected patients, although its precise role in resistance remains to be clarified.
Other ABCC subfamily transporters and related multidrug resistance proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O15440; product specifications from Assay Genie datasheets.