TBXAS1
TBXAS1 (thromboxane A synthase 1, also known as CYP5, CYP5A1, THAS, TXS, TXAS or TS) is an endoplasmic reticulum membrane enzyme that converts prostaglandin H2 to thromboxane A2, a potent inducer of blood vessel constriction and platelet aggregation. Browse Assay Genie's current range of TBXAS1 research reagents below, or read on for the biology behind the target.
TBXAS1 catalyses the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent inducer of blood vessel constriction and platelet aggregation. The enzyme also cleaves PGH2 to 12-hydroxy-heptadecatrienoic acid (12-HHT) and malondialdehyde, which is known to act as a mediator of DNA damage. 12-HHT and malondialdehyde are formed stoichiometrically in the same amounts as TXA2. In addition, TBXAS1 shows dehydratase activity towards (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE), producing 15-KETE and 15-HETE.
The TBXAS1 gene encodes a member of the cytochrome P450 superfamily. Cytochrome P450 proteins are monooxygenases that catalyse many reactions in drug metabolism and in the synthesis of cholesterol, steroids and other lipids. TBXAS1 is placed in the superfamily on the basis of sequence similarity rather than functional similarity. HGNC classifies it within cytochrome P450 family 5 and the hydroperoxy icosatetraenoate dehydratases. The protein is located in the endoplasmic reticulum membrane. Alternatively spliced transcript variants encoding different isoforms have been reported for this gene, and mouse and rat each have a Tbxas1 ortholog.
The enzyme plays a role in several pathophysiological processes, including haemostasis, cardiovascular disease and stroke. Its earlier gene names, thromboxane A synthase 1 (platelet, cytochrome P450, subfamily V) and thromboxane A synthase 1 (platelet), reflect its platelet association and its P450 classification, and explain the CYP5 and CYP5A1 aliases. Researchers studying the TBXAS1 protein often examine it alongside the wider prostaglandin and thromboxane signalling pathway.
TBXAS1 produces thromboxane A2, a potent inducer of platelet aggregation, and has a role in haemostasis.
The enzyme generates a potent vasoconstrictor and is implicated in cardiovascular disease.
TBXAS1 is reported to play a role in stroke, linking thromboxane synthesis to cerebrovascular pathophysiology.
TBXAS1 cleaves PGH2 to TXA2, 12-HHT and malondialdehyde, and dehydrates 15(S)-HPETE to 15-KETE and 15-HETE.
TBXAS1 converts prostaglandin H2 to thromboxane A2, a potent inducer of blood vessel constriction and platelet aggregation. It also cleaves PGH2 to 12-HHT and malondialdehyde, and acts as a dehydratase on 15(S)-HPETE, producing 15-KETE and 15-HETE.
Aliases include CYP5, CYP5A1, THAS, TXS, TXAS and TS, and the protein is also called thromboxane-A synthase. Earlier HGNC names referred to thromboxane A synthase 1 (platelet, cytochrome P450, subfamily V). Alternatively spliced transcript variants encode different isoforms.
Not entirely. It belongs to the cytochrome P450 superfamily on sequence similarity rather than functional similarity, since most P450s are monooxygenases. Mouse and rat both have a Tbxas1 ortholog; check the datasheet of any product for its validated reactivity.
Other cytochrome P450 family members, prostaglandin pathway enzymes and thromboxane signalling targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P24557; product specifications from Assay Genie datasheets.