TPK1
TPK1 (thiamin pyrophosphokinase 1, also known as HTPK1 and PP20) is an enzyme that phosphorylates thiamine to produce thiamine pyrophosphate (TPP). In doing so it links TPP biosynthesis to pyrimidine metabolism. Browse Assay Genie's current range of TPK1 research reagents below, or read on for the biology behind the target.
TPK1 catalyses the phosphorylation of thiamine to thiamine pyrophosphate (TPP), using UTP as the phosphate donor. This ties the biosynthesis of TPP to pyrimidine metabolism. Although the enzyme can also phosphorylate thiamine using ATP and CTP in vitro, it does so with significantly lower efficiency, and there is no evidence that this is physiologically relevant.
TPP is a cofactor of the mitochondrial pyruvate dehydrogenase. By producing it, TPK1 indirectly regulates pyruvate oxidation and lipogenesis. The NCBI Gene summary also describes TPP as a cofactor for some enzymes of the glycolytic and energy production pathways, which places TPK1 in core energy metabolism.
The protein belongs to the thiamine pyrophosphokinase family and functions as a homodimer. The human TPK1 gene is also known by the aliases HTPK1 and PP20, and the mouse ortholog is Tpk1. Defects in the TPK1 gene are a cause of thiamine metabolism dysfunction syndrome-5, which makes the enzyme relevant to studies of inherited disorders of thiamine metabolism.
TPK1 converts thiamine to the active cofactor thiamine pyrophosphate, making it a central enzyme for studying how cells generate TPP.
TPP is a cofactor for enzymes of glycolytic and energy production pathways, so TPK1 activity connects to pyruvate oxidation and cellular energy supply.
Defects in the TPK1 gene cause thiamine metabolism dysfunction syndrome-5, making it a focus for research into thiamine-related disorders.
Because TPK1 uses UTP and influences pyruvate dehydrogenase activity, it links pyrimidine metabolism with pyruvate oxidation and lipogenesis.
TPK1 catalyses the phosphorylation of thiamine to thiamine pyrophosphate (TPP), using UTP. TPP is a cofactor of the mitochondrial pyruvate dehydrogenase, so TPK1 indirectly regulates pyruvate oxidation and lipogenesis. It also links TPP biosynthesis to pyrimidine metabolism.
The HGNC full name is thiamin pyrophosphokinase 1, and UniProt lists the protein as thiamine pyrophosphokinase 1. Aliases include HTPK1 and PP20. The enzyme carries the EC number 2.7.6.- and belongs to the thiamine pyrophosphokinase family.
Yes. According to the NCBI Gene summary, defects in the TPK1 gene are a cause of thiamine metabolism dysfunction syndrome-5. A mouse ortholog, Tpk1, exists; check the datasheet of any product for its validated reactivity.
Other thiamine-metabolism enzymes, pyruvate dehydrogenase components and thiamine transporters with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9H3S4; product specifications from Assay Genie datasheets.