NR1H4
NR1H4 is a ligand-activated transcription factor of the nuclear hormone receptor family that acts as the receptor for bile acids. It is also known as FXR, RIP14, HRR1 and HRR-1, and regulates genes involved in bile acid synthesis and transport. Browse Assay Genie's current range of NR1H4 research reagents below, or read on for the biology behind the target.
NR1H4 (nuclear receptor subfamily 1 group H member 4), commonly called FXR, is a ligand-activated transcription factor that functions as a receptor for bile acids. When bound to bile acids, the protein binds DNA and regulates the expression of genes involved in bile acid synthesis and transport. UniProt annotates it as promoting transcriptional activation of target genes including ABCB11/BSEP, NR0B2/SHP, SLC51B/OSTB and FABP6/IBAP.
The annotated activation of several of these targets is inducible by unconjugated bile acids, namely chenodeoxycholic acid (CDCA), cholic acid (ACA) and deoxycholic acid (DCA). For SLC51B/OSTB, induction is described with unconjugated CDCA and DCA. The most efficient isoform, compared with isoforms 1 to 3, is not inducible by taurine- or glycine-amidated CDCA. Alternatively spliced transcript variants encoding different isoforms have been described, so isoform context matters when interpreting NR1H4 gene and protein data.
The protein belongs to the nuclear hormone receptor family and the NR1 subfamily, and is classed by HGNC in nuclear receptor subfamily 1 group H. It localises to the nucleus. The previous HGNC name was nuclear receptor subfamily 1, group H, member 4, and the gene has mouse and rat orthologs, both named Nr1h4. Related family members include NR1H2 and NR1H3, as well as NR0B2, which is itself an NR1H4 target gene.
NR1H4 is the receptor through which bile acids regulate genes controlling their own synthesis and transport.
As a ligand-activated transcription factor, it drives target genes such as ABCB11/BSEP, NR0B2/SHP, SLC51B/OSTB and FABP6/IBAP.
A member of the NR1 subfamily, NR1H4 is studied alongside other nuclear hormone receptors to understand ligand-dependent gene control.
Splice isoforms differ in efficiency and in response to unconjugated versus amidated bile acids, making NR1H4 useful for isoform-level studies.
NR1H4 is a ligand-activated transcription factor that acts as a bile acid receptor. Once bound to bile acids, it binds DNA and regulates genes involved in bile acid synthesis and transport, including ABCB11/BSEP, NR0B2/SHP, SLC51B/OSTB and FABP6/IBAP.
NR1H4 is also known as FXR, RIP14, HRR1 and HRR-1, and UniProt lists the protein name as bile acid receptor. Alternatively spliced transcript variants encode different isoforms, and one is annotated as the most efficient compared with isoforms 1 to 3.
Yes. Mouse and rat both have an ortholog named Nr1h4, so the receptor can be studied in rodent systems. Species cross-reactivity differs between reagents, so check the datasheet of any product for its validated reactivity.
Other nuclear hormone receptors, NR1 subfamily members and bile acid signalling partners with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q96RI1; product specifications from Assay Genie datasheets.