NAPRT
NAPRT (nicotinate phosphoribosyltransferase, also known as NAPRT1 or PP3856) is a cytosolic enzyme that catalyses the first step of NAD biosynthesis from nicotinic acid. By supporting NAD production it helps limit cellular oxidative stress. Browse Assay Genie's current range of NAPRT research reagents below, or read on for the biology behind the target.
NAPRT catalyses the first step in the biosynthesis of NAD from nicotinic acid (niacin). In an ATP-dependent reaction, it joins nicotinate to 5-phospho-D-ribose 1-phosphate to produce beta-nicotinate D-ribonucleotide. This product, nicotinic acid mononucleotide (NaMN), is then converted to nicotinic acid adenine dinucleotide (NaAD) and finally to nicotinamide adenine dinucleotide (NAD).
NAD is a coenzyme in cellular redox reactions and an essential component of many processes in cellular metabolism, including the response to stress. Through its role in NAD biosynthesis, NAPRT helps prevent cellular oxidative stress. The protein localises to the cytosol, and the NAPRT gene has orthologues named Naprt in both mouse and rat.
NAPRT belongs to the NAPRTase family and is classed by HGNC within the ligases and phosphoribosyltransferases gene groups. The gene was previously named nicotinate phosphoribosyltransferase domain containing 1, and the symbols NAPRT1 and PP3856 appear in older literature. Researchers studying NAD metabolism, niacin utilisation and redox balance use NAPRT as a defined entry point into the nicotinic acid route of NAD synthesis.
NAPRT catalyses the first step of NAD synthesis from nicotinic acid, making it a key enzyme for studying this route of NAD production.
Through its role in NAD biosynthesis, NAPRT helps prevent cellular oxidative stress, making it relevant to stress-response research.
NAD acts as a coenzyme in cellular redox reactions, so NAPRT is a useful target for investigating metabolic redox processes.
NAPRT converts nicotinic acid (niacin) to nicotinic acid mononucleotide, placing it at the start of the pathway that utilises dietary niacin.
NAPRT catalyses the first step in NAD biosynthesis from nicotinic acid. It uses ATP to combine nicotinate with 5-phospho-D-ribose 1-phosphate, forming beta-nicotinate D-ribonucleotide. Through NAD production it also helps prevent cellular oxidative stress.
The HGNC-approved name is nicotinate phosphoribosyltransferase. Aliases include NAPRT1 and PP3856, and the gene was previously called nicotinate phosphoribosyltransferase domain containing 1. NCBI also describes the enzyme as nicotinic acid phosphoribosyltransferase. UniProt classes it in the NAPRTase family.
Yes. Orthologues named Naprt are recorded for both mouse and rat, so the protein can be studied in common rodent models. Check the datasheet of any product for its validated reactivity before choosing a reagent for a particular species.
Other NAD biosynthesis enzymes and related metabolic targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q6XQN6; product specifications from Assay Genie datasheets.