MAPK10
MAPK10, also known as JNK3, p493F12, p54bSAPK or PRKM10, is a serine/threonine protein kinase of the MAP kinase family. It acts in the stress-activated SAP/JNK pathway and regulates neuronal proliferation, differentiation, migration and programmed cell death. Browse Assay Genie's current range of MAPK10 research reagents below, or read on for the biology behind the target.
MAPK10 is a serine/threonine-protein kinase that sits in the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signalling pathway. Extracellular stimuli such as pro-inflammatory cytokines or physical stress activate this cascade, in which the dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10. Activation depends on threonine and tyrosine phosphorylation. MAPK10 then phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2, and so regulates AP-1 transcriptional activity.
Beyond transcription control, MAPK10 has several defined substrates and roles. It plays regulatory roles in signalling during neuronal apoptosis, phosphorylates the neuronal microtubule regulator STMN2, and phosphorylates the amyloid-beta precursor protein (APP) in the regulation of APP signalling during neuronal differentiation. It participates in neurite growth in spiral ganglion neurons, and it phosphorylates the CLOCK-BMAL1 heterodimer, contributing to photic regulation of the circadian clock. It also phosphorylates JUND, and this is inhibited in the presence of MEN1. The kinase has been found in the cytoplasm, nucleus, mitochondrion and membrane fractions.
The MAPK10 gene encodes a member of the MAP kinase family, within the protein kinase superfamily and the CMGC Ser/Thr kinase group. HGNC places it in the JNK family (MAPK8-10). MAP kinases integrate multiple biochemical signals and take part in proliferation, differentiation, transcription regulation and development. MAPK10 is specifically expressed in a subset of neurons in the nervous system, and targeted deletion in mice suggests a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encode different isoforms, and translational readthrough has been reported to give an additional C-terminally extended isoform.
MAPK10 regulates signalling during neuronal apoptosis, and mouse deletion studies point to a role in stress-induced neuronal cell death.
MAPK10 is activated by MAP2K4 and MAP2K7 and phosphorylates JUN and ATF2, making it a tool for studying AP-1 regulation.
MAPK10 phosphorylates APP and STMN2 and participates in neurite growth in spiral ganglion neurons.
MAPK10 phosphorylates the CLOCK-BMAL1 heterodimer and contributes to photic regulation of the circadian clock.
MAPK10 is a serine/threonine kinase activated by MAP2K4 and MAP2K7 in the SAP/JNK pathway. It phosphorylates transcription factors such as JUN and ATF2, regulating AP-1 activity, and has roles in neuronal proliferation, differentiation, migration and apoptosis.
MAPK10 is also called JNK3, p493F12, p54bSAPK and PRKM10. Alternatively spliced transcript variants encode different isoforms, and translational readthrough has been reported to produce an additional C-terminally extended isoform, according to NCBI Gene.
Mouse and rat both have a Mapk10 ortholog. Mouse gene deletion suggests a role for the kinase in stress-induced neuronal apoptosis. Check the datasheet of any product for its validated reactivity.
Other MAP kinases, MAPK-activated protein kinases and JNK pathway partners with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P53779; product specifications from Assay Genie datasheets.