PCYOX1
PCYOX1 (prenylcysteine oxidase 1, also known as KIAA0908 and PCL1) is a lysosomal enzyme that cleaves the thioether bond of free prenyl-L-cysteines. It acts in the final step of prenylated protein degradation. Browse Assay Genie's current range of PCYOX1 research reagents below, or read on for the biology behind the target.
PCYOX1 is a prenylcysteine oxidase that cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine. Its activity is restricted to free prenylcysteines: it does not act on prenylcysteine residues within prenylated proteins or peptides. This specificity places the enzyme at the end of the degradation route, where it breaks down prenylcysteines only after the parent protein has itself been degraded.
Prenylcysteine is released during the degradation of prenylated proteins. According to the NCBI Gene summary, PCYOX1 catalyses the degradation of prenylcysteine to yield free cysteine and a hydrophobic isoprenoid product, a function described by Tschantz and colleagues in 1999. The protein is found in the lysosome, consistent with a role in the turnover of protein breakdown products.
The PCYOX1 gene encodes a member of the prenylcysteine oxidase family, which HGNC classifies within the flavoproteins. The enzyme carries the EC number 1.8.3.5. The gene has also been referred to as KIAA0908 and PCL1, and mouse and rat each have an ortholog named Pcyox1. Researchers studying prenylation, isoprenoid metabolism and lysosomal protein catabolism use the PCYOX1 protein as a defined point of reference for the closing step of this pathway.
PCYOX1 degrades prenylcysteines released after prenylated proteins are broken down, making it relevant to studies of how prenylated proteins are turned over.
The enzyme converts prenylcysteine into free cysteine and a hydrophobic isoprenoid product, so it is of interest in work on isoprenoid handling.
As a lysosomal enzyme acting at the final step of prenylated protein degradation, PCYOX1 is a useful subject for lysosomal catabolism research.
PCYOX1 belongs to the prenylcysteine oxidase family of flavoproteins and is studied for its substrate specificity towards free prenylcysteines.
PCYOX1 cleaves the thioether bond of free prenyl-L-cysteines, including farnesylcysteine and geranylgeranylcysteine. It does not act on prenylcysteine within prenylated proteins or peptides. It performs the final step in prenylated protein degradation, after the protein itself has been degraded.
PCYOX1 is the HGNC symbol for prenylcysteine oxidase 1. The gene has also been called KIAA0908 and PCL1. The enzyme is classified under EC 1.8.3.5 and belongs to the prenylcysteine oxidase family of flavoproteins.
Yes. Mouse and rat each have an ortholog named Pcyox1, so the gene is conserved across these common model species. Check the datasheet of any product for its validated reactivity.
Other flavoproteins and enzymes involved in protein prenylation and lysosomal degradation may be of interest alongside PCYOX1.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9UHG3; product specifications from Assay Genie datasheets.