PIP4K2C
PIP4K2C (phosphatidylinositol-5-phosphate 4-kinase type 2 gamma) is a phosphoinositide kinase with low enzymatic activity that negatively regulates insulin signalling. It is also known as PIP5K2C and FLJ22055. Browse Assay Genie's current range of PIP4K2C research reagents below, or read on for the biology behind the target.
PIP4K2C is a phosphatidylinositol 5-phosphate 4-kinase with low enzymatic activity. UniProt notes that it may act as a GTP sensor, since its GTP-dependent kinase activity is higher than its ATP-dependent activity. The PIP4K enzymes negatively regulate insulin signalling through a catalytic-independent mechanism: they interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis, as well as the insulin-dependent conversion of this lipid to PtdIns(3,4,5)P3.
NCBI Gene annotation describes the protein as enabling 1-phosphatidylinositol-4-phosphate 5-kinase activity and identical protein binding. It is involved in the biosynthesis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate, in negative regulation of the insulin receptor signalling pathway and in positive regulation of autophagosome assembly. It is located in the autophagosome and cytosol, and UniProt lists the endoplasmic reticulum and cytoplasm as subcellular locations.
The PIP4K2C gene belongs to the HGNC group of phosphatidylinositol-5-phosphate 4-kinase type 2 family members. Earlier names include phosphatidylinositol-4-phosphate 5-kinase, type II, gamma, and the former symbol PIP5K2C. Mouse and rat both carry an ortholog named Pip4k2c, which allows the PIP4K2C protein to be studied in common model systems. Researchers working on the PIP4K2C gene often study it alongside the other PIP4K and PIP5K lipid kinases.
PIP4K2C negatively regulates insulin signalling by suppressing PIP5K-mediated PtdIns(4,5)P2 synthesis and its insulin-dependent conversion to PtdIns(3,4,5)P3.
The enzyme contributes to the biosynthetic process of phosphatidylinositol 4,5-bisphosphate, making it relevant to studies of phosphoinositide lipid kinases.
NCBI Gene annotation links PIP4K2C to positive regulation of autophagosome assembly, and places it in the autophagosome.
Its higher GTP-dependent than ATP-dependent kinase activity suggests a possible role as a GTP sensor, a point of interest for enzymology.
PIP4K2C is a phosphatidylinositol 5-phosphate 4-kinase with low enzymatic activity. It negatively regulates insulin signalling through a catalytic-independent mechanism, interacting with PIP5Ks and suppressing PtdIns(4,5)P2 synthesis. It may also act as a GTP sensor, as it shows higher GTP-dependent activity.
The HGNC name is phosphatidylinositol-5-phosphate 4-kinase type 2 gamma. Aliases and previous symbols include PIP5K2C and FLJ22055. Earlier names were phosphatidylinositol-4-phosphate 5-kinase, type II, gamma, and phosphatidylinositol-5-phosphate 4-kinase, type II, gamma.
Yes. Both mouse and rat have an ortholog named Pip4k2c, so the protein can be studied in these model organisms. Check the datasheet of any product for its validated reactivity.
Other phosphoinositide kinases and similarly named targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8TBX8; product specifications from Assay Genie datasheets.