STK24
STK24 (serine/threonine kinase 24, also known as MST-3, MST3, MST3B, STK3 and STE20) is a STE20-subfamily protein kinase that acts on serine and threonine residues. It promotes apoptosis in response to stress and caspase activation, and sits upstream of MAPK signalling. Browse Assay Genie's current range of STK24 research reagents below, or read on for the biology behind the target.
STK24 is a serine/threonine-protein kinase that promotes apoptosis in response to stress stimuli and caspase activation. It mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1 and JNK2 (MAPK8 and MAPK9) and of p38 family kinases (MAPK11, MAPK12, MAPK13 and MAPK14). It also contributes to a staurosporine-induced, caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG, and the DNase activity associated with ENDOG.
Beyond cell death, STK24 phosphorylates STK38L on Thr-442 and stimulates its kinase activity. Together with STK26, it negatively regulates Golgi reorientation in polarised cell migration after RHO activation. It also regulates cellular migration by phosphorylating PTPN12 and inhibiting its activity, and it may influence PXN phosphorylation through PTPN12. It may be a key regulator of axon regeneration in the optic nerve and radial nerve.
The STK24 protein is cleaved into two chains by caspases. The N-terminal fragment, MST3/N, translocates to the nucleus and promotes programmed cell death. STK24 is part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. These regulate protein (de)phosphorylation and several signalling pathways, including Hippo, MAPK, nuclear receptor and cytoskeleton remodelling. Different STRIPAK complexes take part in cell growth, differentiation, apoptosis, metabolism and immune regulation.
The STK24 gene belongs to the STK24/STK25/STK26 kinase family and the STE Ser/Thr protein kinase family, STE20 subfamily. Its previous name, serine/threonine kinase 24 (Ste20, yeast homolog), reflects this lineage. A pseudogene exists on chromosome X, and alternative splicing produces multiple transcript variants.
STK24 promotes apoptosis after stress and caspase activation, including oxidative-stress-induced death through JNK and p38 phosphorylation.
As part of STRIPAK complexes, STK24 links to regulation of Hippo, MAPK, nuclear receptor and cytoskeleton remodelling pathways.
STK24 regulates migration through PTPN12 and PXN phosphorylation and, with STK26, controls Golgi reorientation in polarised cells.
STK24 may be a key regulator of axon regeneration in the optic nerve and radial nerve.
STK24 is a serine/threonine kinase that promotes apoptosis after stress stimuli and caspase activation. It modulates JNK and p38 phosphorylation, regulates AIFM1 and ENDOG nuclear translocation, and phosphorylates STK38L and PTPN12. It also influences cell migration and is part of STRIPAK complexes.
STK24 is also called MST-3, MST3, MST3B, STK3 and STE20. Its former HGNC name was serine/threonine kinase 24 (Ste20, yeast homolog). Alternative splicing gives multiple transcript variants, and caspase cleavage yields an N-terminal fragment, MST3/N, that enters the nucleus.
Yes. Mouse and rat both have an Stk24 ortholog of the human gene. Human STK24 belongs to the STE20 subfamily of the STE Ser/Thr protein kinase family; check the datasheet of any product for its validated reactivity.
Other serine/threonine kinases, including STK24 family partner STK26 and related STE20-type kinases, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9Y6E0; product specifications from Assay Genie datasheets.