USP39
USP39 (ubiquitin specific peptidase 39, also known as SAD1, CGI-21 and SNRNP65) is a nuclear deubiquitinating enzyme and a component of the U4/U6-U5 tri-snRNP. It links pre-mRNA splicing with DNA repair, cell cycle control and immune signalling. Browse Assay Genie's current range of USP39 research reagents below, or read on for the biology behind the target.
USP39 is a deubiquitinating enzyme that acts in many cellular processes, including the cellular antiviral response, epithelial morphogenesis, DNA repair and B-cell development. It is a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the precatalytic spliceosome, and so contributes to pre-mRNA splicing. NCBI Gene describes it as part of the U4/U6 x U5 tri-snRNP complex, involved in spliceosomal complex assembly and located in the nucleoplasm. It is predicted to have cysteine-type deubiquitinase activity and zinc ion binding activity.
Beyond splicing, USP39 regulates specific substrates and transcripts. It controls immunoglobulin gene rearrangement in a spliceosome-dependent manner by modulating chromatin interactions at the Igh locus, which is essential for B-cell development. It regulates AURKB mRNA levels and thereby cytokinesis and the spindle checkpoint. In response to DNA damage, it deubiquitinates and stabilises CHK2, regulating apoptosis and the G2/M checkpoint, and it controls recruitment of XRCC4/LIG4 to double-strand breaks for non-homologous end-joining repair.
USP39 also shapes signalling pathways. It supports type I IFN signalling and antiviral activity by stabilising STAT1 and decreasing its 'Lys-6'-linked ubiquitination, contributes to non-canonical Wnt signalling during epidermal differentiation, and negatively regulates NF-kappa-B activation by removing 'Lys-48'-linked ubiquitin from NFKBIA. The USP39 gene belongs to the peptidase C19 family and the HGNC ubiquitin specific peptidase and tri-snRP complex gene groups. The symbol was formerly written as ubiquitin specific protease 39, and a mouse ortholog, Usp39, is recognised.
USP39 is a tri-snRNP component involved in spliceosomal complex assembly, making it relevant to studies of precatalytic spliceosome organisation.
USP39 stabilises CHK2 and controls XRCC4/LIG4 recruitment to double-strand breaks, linking it to checkpoint control and non-homologous end-joining.
USP39 stabilises STAT1 in type I IFN signalling and restrains NF-kappa-B activation through NFKBIA deubiquitination.
USP39 regulates Igh locus rearrangement in B-cell development and AURKB mRNA levels, affecting cytokinesis and the spindle checkpoint.
USP39 is a deubiquitinating enzyme and a component of the U4/U6-U5 tri-snRNP involved in pre-mRNA splicing. It also stabilises CHK2 and STAT1, supports DNA repair by non-homologous end-joining, regulates AURKB mRNA, and negatively regulates NF-kappa-B activation via NFKBIA.
USP39 is also known as SAD1, CGI-21 and SNRNP65. HGNC lists the full name as ubiquitin specific peptidase 39, previously ubiquitin specific protease 39, and UniProt calls the protein ubiquitin carboxyl-terminal hydrolase 39. It carries the enzyme classification EC 3.4.19.12.
A mouse ortholog, Usp39, is recognised. UniProt links USP39 to B-cell development through regulation of immunoglobulin gene rearrangement at the Igh locus, and to antiviral responses through STAT1 stabilisation. Check the datasheet of any product for its validated reactivity.
Other ubiquitin specific peptidases and deubiquitinating enzymes with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q53GS9; product specifications from Assay Genie datasheets.