MAPK15
MAPK15 (mitogen-activated protein kinase 15, also known as ERK8 and ERK7) is an atypical MAP kinase that regulates autophagy, ciliogenesis, protein trafficking and genome integrity. It acts mainly in a kinase activity-dependent manner. Browse Assay Genie's current range of MAPK15 research reagents below, or read on for the biology behind the target.
MAPK15 is an atypical mitogen-activated protein kinase that controls basal and starvation-induced autophagy through its interaction with GABARAP, MAP1LC3B and GABARAPL1, leading to autophagosome formation, SQSTM1 degradation and reduced inhibitory phosphorylation of MAP1LC3B. It also regulates primary cilium formation and the localisation of ciliary proteins involved in cilium structure, transport and signalling. At the Golgi, it prevents the relocation of sugar-adding enzymes to the endoplasmic reticulum, and upon amino-acid starvation it mediates disassembly of transitional endoplasmic reticulum sites and inhibition of secretion.
Further roles are described in genome maintenance and signalling. Binding to chromatin activates MAPK15, which then interacts with PCNA and protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA. The kinase regulates dopamine transporter activity and expression via RhoA, and in response to H(2)O(2) it phosphorylates ELAVL1, exposing PDCD4 mRNA to miR-21 and causing its degradation. It interacts with ESRRA, promoting relocation from the nucleus to the cytoplasm and preventing its transcriptional activity. In vitro it phosphorylates FOS and MBP. It also acts as a negative regulator of growth independently of its kinase activity, and contributes to microtubule organisation and meiotic progression in oocytes, fertilised eggs and early embryos.
The MAPK15 gene encodes a member of the CMGC Ser/Thr protein kinase family, MAP kinase subfamily, classed by HGNC among the atypical mitogen-activated protein kinases. Orthologues are named Mapk15 in mouse and rat. UniProt records localisation to the cilium basal body, tight junctions, centrioles, autophagosomes, Golgi apparatus, nucleus, spindle and cytoplasm. NCBI Gene describes it as a biomarker of breast cancer.
MAPK15 interacts with GABARAP, MAP1LC3B and GABARAPL1 to control autophagosome formation and SQSTM1 degradation under basal and starvation conditions.
MAPK15 regulates primary cilium formation and the localisation of ciliary proteins involved in cilium structure, transport and signalling.
MAPK15 restricts Golgi enzyme relocation to the endoplasmic reticulum and mediates transitional ER site disassembly and secretion inhibition on starvation.
MAPK15 protects PCNA from MDM2-mediated degradation, and NCBI Gene lists it as a biomarker of breast cancer.
MAPK15 is an atypical MAP kinase that regulates autophagy, ciliogenesis, protein trafficking and secretion, and genome integrity, largely depending on its kinase activity. It also acts as a negative regulator of growth independently of kinase activity and contributes to meiotic progression in oocytes.
Yes. ERK8 and ERK7 are aliases of MAPK15, the HGNC-approved symbol for mitogen-activated protein kinase 15. Literature may use any of these names for the same gene and protein, so check all of them when searching.
Mouse and rat both have a Mapk15 orthologue. NCBI Gene describes MAPK15 as a biomarker of breast cancer, and UniProt links it to PCNA protection and genome integrity. Check the datasheet of any product for its validated reactivity.
Other MAP kinases, atypical MAPK family members and MAPK-activated kinases with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8TD08; product specifications from Assay Genie datasheets.