ODF2
ODF2 (outer dense fibre of sperm tails 2, also known as ODF84 and CT134) is a major component of the outer dense fibres of the sperm tail. It also acts as a scaffold protein at the appendages of mother centrioles in somatic cells. Browse Assay Genie's current range of ODF2 research reagents below, or read on for the biology behind the target.
ODF2 appears to be a major component of the outer dense fibres (ODF) of the sperm tail. These filamentous structures lie on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail. They are thought to help maintain the passive elastic structures and elastic recoil of the tail, and ODF2 may have a modulating influence on sperm motility. According to the NCBI Gene summary, the fibres also protect the tail from shear forces during epididymal transport and ejaculation, and defects in them lead to abnormal sperm morphology and infertility.
Outside the sperm tail, ODF2 functions as a general scaffold protein that localises specifically to the distal and subdistal appendages of mother centrioles. It is a component of the centrosome matrix and is required for the localisation of PLK1 and NIN to the centrosomes. It is also required for the formation and/or maintenance of normal CETN1 assembly. Reported subcellular locations include the centrosome, centriole, spindle pole, cilium and flagellum.
The ODF2 gene encodes one of the major outer dense fibre proteins, and alternative splicing produces multiple transcript variants. The longer transcripts, known as 'Cenexins', encode proteins with a C-terminal extension. These are differentially targeted to somatic centrioles and are thought to be crucial for the formation of microtubule organizing centres. ODF2 belongs to the ODF2 family, and the protein has also been indexed under the cancer/testis designation CT134 and the previous name outer dense fibre of sperm tails 2.
ODF2 is a major outer dense fibre protein linked to sperm tail elasticity, protection from shear forces and sperm motility.
Defects in outer dense fibres lead to abnormal sperm morphology and infertility, making ODF2 relevant to studies of sperm structure.
ODF2 scaffolds the distal and subdistal appendages of mother centrioles and is needed for PLK1 and NIN localisation at centrosomes.
Longer ODF2 transcripts, the Cenexins, target somatic centrioles and are thought to be crucial for forming microtubule organizing centres.
ODF2 is a major component of sperm tail outer dense fibres, which may help maintain tail elasticity and recoil and may modulate motility. It is also a scaffold protein at mother centriole appendages, required for PLK1 and NIN localisation to centrosomes and for normal CETN1 assembly.
ODF2 is also known as ODF84 and CT134, and its previous name is outer dense fibre of sperm tails 2. Alternative splicing gives multiple transcript variants. The longer ones, called 'Cenexins', encode a C-terminal extension and are differentially targeted to somatic centrioles.
Mouse and rat both have an Odf2 ortholog. According to the NCBI Gene summary, defects in the outer dense fibres lead to abnormal sperm morphology and infertility. Check the datasheet of any product for its validated reactivity.
Centrosome and centriole proteins, and other outer dense fibre components that relate to ODF2 biology.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q5BJF6; product specifications from Assay Genie datasheets.