CNOT7
CNOT7 (CCR4-NOT transcription complex subunit 7, also known as CAF1) is a catalytic subunit of the CCR4-NOT complex, one of the major cellular mRNA deadenylases. It has 3'-5' poly(A) exoribonuclease activity and links mRNA turnover to cell proliferation control. Browse Assay Genie's current range of CNOT7 research reagents below, or read on for the biology behind the target.
CNOT7 is a catalytic component of the CCR4-NOT complex, which is one of the major cellular mRNA deadenylases. The protein shows 3'-5' poly(A) exoribonuclease activity on synthetic poly(A) RNA substrates. Through the complex, it is linked to bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its function appears to be partially redundant with that of CNOT8.
The CNOT7 protein belongs to the CAF1 family and is assigned to the HGNC gene group for the CCR4-NOT transcription complex. It localises to the nucleus and to the cytoplasm, where it is found in P-bodies and cytoplasmic ribonucleoprotein granules. The CNOT7 gene is on chromosome 8p22, and alternative splicing gives rise to multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and X. The mouse ortholog is Cnot7.
CNOT7 associates with members of the BTG family, such as TOB1 and BTG2, and is required for their anti-proliferative activity. According to the NCBI Gene summary, it binds the anti-proliferative protein B-cell translocation protein 1, and this binding is driven by phosphorylation of that partner. It triggers signalling events in cell division that change proliferation linked to cell-cell contact. The protein also downregulates the innate immune response, so it has been proposed as a therapeutic target for enhancing antimicrobial activity. The previous name was CCR4-NOT transcription complex, subunit 7, and CAF1 is a recognised alias.
CNOT7 is a catalytic subunit of a major cellular mRNA deadenylase, making it central to studies of bulk mRNA degradation.
The CCR4-NOT complex contributes to miRNA-mediated repression and translational repression during initiation, so CNOT7 is relevant to post-transcriptional silencing.
CNOT7 associates with TOB1 and BTG2 and is required for their anti-proliferative activity, linking it to the regulation of cell division.
CNOT7 downregulates the innate immune response, and it has been suggested as a therapeutic target for boosting antimicrobial activity.
CNOT7 is a catalytic component of the CCR4-NOT complex, a major cellular mRNA deadenylase. It has 3'-5' poly(A) exoribonuclease activity. The complex contributes to bulk mRNA degradation, miRNA-mediated repression, translational repression and general transcription regulation. CNOT7 is also required for the anti-proliferative activity of BTG family proteins.
CNOT7 is also known as CAF1, and its earlier name was CCR4-NOT transcription complex, subunit 7. It belongs to the CAF1 family. Alternative splicing of the gene produces multiple transcript variants, and related pseudogenes have been identified on chromosomes 1 and X.
A mouse ortholog, Cnot7, is recorded for the human gene. Within the CCR4-NOT complex, the function of CNOT7 seems to be partially redundant with that of CNOT8. Check the datasheet of any product for its validated reactivity.
Other CCR4-NOT complex subunits, BTG family proteins and mRNA-turnover factors related to CNOT7 biology.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9UIV1; product specifications from Assay Genie datasheets.