CD73
CD73 (5'-nucleotidase ecto, encoded by NT5E) is a plasma membrane enzyme that hydrolyses nucleotide monophosphates, preferring AMP, to release phosphate and the corresponding nucleoside. It is also known as eN, eNT, CALJA and NT5. Browse Assay Genie's current range of CD73 research reagents below, or read on for the biology behind the target.
CD73 catalyses the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside. AMP is the preferred substrate, and the enzyme shows a preference for ribonucleotide monophosphates over their deoxyribose equivalents. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN. At the cell surface, this activity converts extracellular nucleotides into membrane-permeable nucleosides.
The NT5E gene encodes a plasma membrane protein that belongs to the 5'-nucleotidase family. HGNC classifies it within the CD molecules and the 5'-nucleotidases gene groups. Several EC numbers are assigned to the enzyme, reflecting its activity towards a range of nucleotide substrates. Two transcript variants encoding different isoforms have been reported for the CD73 gene. Mouse and rat both have an orthologous gene, Nt5e.
CD73 is used as a determinant of lymphocyte differentiation, which accounts for its CD designation. Defects in the gene can lead to calcification of joints and arteries. Previous names include 5' nucleotidase (CD73) and 5'-nucleotidase, ecto (CD73), and the protein is also referred to as eN or eNT. Researchers studying extracellular nucleotide metabolism, lymphocyte biology or vascular calcification often investigate CD73 expression and activity, using tools such as a CD73 antibody or CD73 ELISA kit.
CD73 converts extracellular nucleotides, with AMP preferred, into membrane-permeable nucleosides, making it a key enzyme for studying nucleotide handling at the cell surface.
The protein is used as a determinant of lymphocyte differentiation, so it serves as a marker in studies of lymphocyte populations.
Defects in NT5E can lead to calcification of joints and arteries, linking CD73 to research on ectopic mineralisation.
Activity towards AMP, IMP, UMP, GMP, CMP, NAD and NMN, with a ribonucleotide preference, makes CD73 useful for studies of phosphohydrolase specificity.
CD73 is a cell membrane 5'-nucleotidase that hydrolyses nucleotide monophosphates to release inorganic phosphate and the matching nucleoside. AMP is the preferred substrate. At the cell surface it converts extracellular nucleotides into membrane-permeable nucleosides, and it is used as a determinant of lymphocyte differentiation.
CD73 is encoded by NT5E and is also called 5'-nucleotidase, ecto, eN, eNT, CALJA and NT5. Earlier names include 5' nucleotidase (CD73). Two transcript variants encoding different isoforms have been described for the gene.
Defects in the NT5E gene can lead to calcification of joints and arteries. Orthologous genes named Nt5e exist in mouse and rat, so rodent models can be used to study the protein. Check the datasheet of any product for its validated reactivity.
Another 5'-nucleotidase family member and other NT-prefixed targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P21589; product specifications from Assay Genie datasheets.