YTHDF1
YTHDF1 (YTH N6-methyladenosine RNA binding protein F1, also known as C20orf21 and FLJ20391) is a cytoplasmic reader of N6-methyladenosine (m6A) modifications in mRNA. It recognises m6A-containing transcripts and regulates their stability and fate. Browse Assay Genie's current range of YTHDF1 research reagents below, or read on for the biology behind the target.
YTHDF1 specifically recognises and binds mRNAs that carry N6-methyladenosine (m6A), a modification found at internal sites of mRNAs and some non-coding RNAs that influences mRNA stability and processing. UniProt annotates YTHDF1 as a regulator of mRNA stability that promotes degradation of m6A-containing transcripts through interaction with the CCR4-NOT complex. NCBI Gene also describes ribosome binding activity and a role in positive regulation of translational initiation, alongside mRNA destabilisation.
The protein is a member of the YTHDF family, YTHDF1 subfamily, and is classed by HGNC in the group of YTH domain containing N6-methyladenosine readers. The paralogs YTHDF1, YTHDF2 and YTHDF3 share m6A-containing mRNA targets and are reported to act redundantly in mRNA degradation and cellular differentiation. YTHDF1 is found in the cytoplasm, including P-bodies and stress granules. It promotes the formation of these membraneless compartments by liquid-liquid phase separation: mRNAs carrying multiple m6A residues act as a multivalent scaffold that brings together the disordered regions of YTHDF proteins, and the resulting mRNA-YTHDF complexes partition into P-bodies, stress granules or neuronal RNA granules.
Functional roles reported for YTHDF1 include facilitating learning and memory formation in the hippocampus by binding m6A-containing neuronal mRNAs, and regulating axon guidance through m6A-containing ROBO3 transcripts. It also acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells, which in tumorigenesis limits the anti-tumour response by reducing the efficiency of tumour-antigen cross-presentation. The YTHDF1 gene is implicated in colorectal cancer, and the mouse ortholog is Ythdf1. Earlier names include YTH domain family 1 and YTH N6-methyladenosine RNA binding protein 1.
YTHDF1 is a reader that recognises m6A-containing mRNAs, making it central to studies of how this modification controls mRNA stability and processing.
YTHDF1 promotes degradation of m6A mRNAs via the CCR4-NOT complex and is linked to translational initiation and ribosome binding.
Binding to polymethylated mRNAs drives liquid-liquid phase separation, placing YTHDF1 in P-bodies, stress granules and neuronal RNA granules.
YTHDF1 supports hippocampal learning and axon guidance, limits antigen cross-presentation in dendritic cells, and is implicated in colorectal cancer.
YTHDF1 binds m6A-containing mRNAs and regulates their stability, promoting degradation through the CCR4-NOT complex. It also contributes to translational initiation, stress granule assembly and phase separation of mRNA-protein complexes into P-bodies and stress granules. Check the datasheet of any product for its validated reactivity.
YTHDF1 is also known as YTH domain-containing family protein 1, C20orf21 and FLJ20391. Earlier names include YTH domain family, member 1, and YTH N6-methyladenosine RNA binding protein 1. It belongs to the YTHDF family, YTHDF1 subfamily.
NCBI Gene lists YTHDF1 as implicated in colorectal cancer, and UniProt describes a role in limiting anti-tumour responses by suppressing antigen cross-presentation in dendritic cells. The mouse ortholog is Ythdf1, and YTHDF1, YTHDF2 and YTHDF3 share m6A mRNA targets.
Other m6A readers, writers and erasers, and pathway partners linked to YTHDF1 biology.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9BYJ9; product specifications from Assay Genie datasheets.