RAP1GAP
RAP1GAP (RAP1 GTPase activating protein, also known as RAP1GAP1, RAP1GAPII, RAP1GA1 and KIAA0474) is a GTPase-activating protein that down-regulates the ras-related protein RAP1. It returns RAP1 to its inactive GDP-bound state. Browse Assay Genie's current range of RAP1GAP research reagents below, or read on for the biology behind the target.
RAP1GAP acts as a negative regulator of RAP1 signalling. RAP1 is a ras-related molecular switch that cycles between an inactive GDP-bound form and an active GTP-bound form. RAP1GAP promotes hydrolysis of the bound GTP, which returns RAP1 to the inactive state. UniProt describes it as a GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
The opposing activity comes from guanine nucleotide exchange factors (GEFs), which activate RAP1 by facilitating conversion from the GDP- to the GTP-bound form. The balance between GEF and GAP activity therefore sets the signalling state of RAP1. Ras subfamily proteins such as RAP1 play key roles in receptor-linked signalling pathways that control cell growth and differentiation, and RAP1 is implicated in cell proliferation, adhesion, differentiation and embryogenesis.
The human RAP1GAP gene encodes Rap1 GTPase-activating protein 1, which UniProt places at the Golgi apparatus membrane. Alternative splicing results in multiple transcript variants encoding distinct proteins. The gene has also been known as RAP1, GTPase activating protein 1, and the mouse ortholog is Rap1gap. Researchers studying RAP1GAP often examine it alongside RAP1 isoforms and the GEFs that oppose its activity.
RAP1GAP is a defined GAP for RAP1, making it a useful component for studying how GTP hydrolysis switches RAP1 to its inactive state.
As a regulator of RAP1, RAP1GAP is relevant to receptor-linked signalling pathways that control cell growth and differentiation.
RAP1 contributes to adhesion and proliferation, so its negative regulator RAP1GAP is studied in these processes.
RAP1 plays a role in embryogenesis, which makes control of its activity by RAP1GAP of interest in developmental studies.
RAP1GAP is a GTPase-activating protein that down-regulates RAP1. It promotes hydrolysis of RAP1-bound GTP, returning RAP1 to its inactive GDP-bound form. Guanine nucleotide exchange factors act in the opposite direction by activating RAP1.
RAP1GAP is also known as RAP1GAP1, RAP1GAPII, RAP1GA1 and KIAA0474. Its previous HGNC name was RAP1, GTPase activating protein 1. Alternative splicing produces multiple transcript variants encoding distinct proteins, so isoform details matter when selecting reagents.
A mouse ortholog, Rap1gap, is recorded for the human gene. This allows comparative work in mouse models of RAP1 signalling. Check the datasheet of any product for its validated reactivity.
Other RAP1 GTPases, related regulators of small GTPase signalling and RAP-family proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P47736; product specifications from Assay Genie datasheets.