GART
GART is a trifunctional enzyme that catalyses three distinct reactions in the de novo inosine monophosphate biosynthetic pathway. It is also known as GARS-AIRS-GART, PRGS and PGFT, and its UniProt name is trifunctional purine biosynthetic protein adenosine-3. Browse Assay Genie's current range of GART research reagents below, or read on for the biology behind the target.
GART is a trifunctional polypeptide that carries out three distinct reactions as part of the de novo inosine monophosphate biosynthetic pathway. Its activities are phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and phosphoribosylaminoimidazole synthetase, and together they are required for de novo purine biosynthesis. Combining these steps in one polypeptide is the defining feature of the protein.
The GART gene is the source of the protein, and its full HGNC name lists the three activities it encodes: phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and phosphoribosylaminoimidazole synthetase. UniProt places the C-terminal section within the GART family. The enzyme is highly conserved in vertebrates, and the mouse ortholog is designated Gart. Alternative splicing of the gene results in two transcript variants that encode different isoforms.
HGNC classifies GART within the purinosome, ATP-grasp fold containing and ligase gene groups. The aliases GARS-AIRS-GART, PRGS and PGFT appear in the literature and in databases, and reflect the multifunctional nature of the protein. Researchers studying nucleotide metabolism, purine synthesis and purinosome organisation use GART as a reference enzyme, whether they work with a GART antibody, a GART ELISA kit or recombinant GART protein.
GART catalyses three reactions in the de novo inosine monophosphate pathway and is required for de novo purine biosynthesis.
HGNC places GART in the purinosome gene group, making it relevant to studies of how purine biosynthetic enzymes are organised.
As an ATP-grasp fold containing ligase with several activities in one polypeptide, GART is a model for multifunctional enzymes.
The enzyme is highly conserved in vertebrates, supporting comparison between human GART and the mouse Gart ortholog.
GART is a trifunctional enzyme that catalyses three distinct reactions in the de novo inosine monophosphate biosynthetic pathway. Its phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and phosphoribosylaminoimidazole synthetase activities are required for de novo purine biosynthesis.
GART is also called GARS-AIRS-GART, PRGS and PGFT, and UniProt lists it as trifunctional purine biosynthetic protein adenosine-3. Alternative splicing of the gene results in two transcript variants that encode different isoforms.
Yes. NCBI Gene describes the enzyme as highly conserved in vertebrates, and the mouse ortholog is Gart. Check the datasheet of any product for its validated reactivity.
Other purine biosynthesis enzymes and nucleotide-metabolism targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P22102; product specifications from Assay Genie datasheets.