SBDS
SBDS (SBDS ribosome maturation factor, also known as CGI-97, SDS, SWDS and SDO1) is a highly conserved protein required for ribosome biogenesis. Together with EFL1, it releases EIF6 from pre-60S ribosomal subunits so that ribosomes become competent for translation. Browse Assay Genie's current range of SBDS research reagents below, or read on for the biology behind the target.
SBDS is required for the assembly of mature ribosomes and for ribosome biogenesis. In the cytoplasm it acts together with elongation factor-like GTPase 1 (EFL1) to trigger the GTP-dependent release of eukaryotic initiation factor 6 (EIF6) from 60S pre-ribosomes. Removing EIF6 activates the ribosome for translation by allowing 80S ribosome assembly, and it also facilitates recycling of EIF6 to the nucleus, where EIF6 is needed for 60S rRNA processing and nuclear export. Consistent with this role, SBDS is required for normal levels of protein synthesis.
The protein belongs to the SDO1/SBDS family and is classed by HGNC among the ribosome biogenesis factors. UniProt records it in the cytoplasm, the nucleolus and nucleoplasm of the nucleus, and at the spindle in the cytoskeleton. The same annotation indicates that SBDS may contribute to cellular stress resistance, the cellular response to DNA damage and cell proliferation. These roles are described as possible rather than established. The mouse and rat orthologues are both named Sbds, which suits comparative work on this SBDS gene.
Mutations in the SBDS gene are associated with the autosomal recessive disorder Shwachman-Bodian-Diamond syndrome, which gave the gene its original name. HGNC also lists the previous name SBDS, ribosome assembly guanine nucleotide exchange factor. NCBI notes that the gene has a closely linked pseudogene located distally, which is worth bearing in mind when designing nucleic acid based experiments.
SBDS is required for assembling mature ribosomes, making it a core factor for studying 60S subunit maturation and the release of EIF6.
By enabling 80S ribosome assembly, SBDS is required for normal levels of protein synthesis and for ribosomes to reach translation competence.
Mutations in SBDS are associated with this autosomal recessive disorder, so the protein is central to research into its molecular basis.
SBDS may play a role in cellular stress resistance, the response to DNA damage and cell proliferation, which remain open research questions.
SBDS is required for ribosome biogenesis. With EFL1 it triggers GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, allowing 80S ribosome assembly and recycling of EIF6 to the nucleus. It is also required for normal levels of protein synthesis.
HGNC lists the aliases and previous symbols CGI-97, FLJ10917, SDS, SWDS and SDO1. Previous names include Shwachman-Bodian-Diamond syndrome and SBDS, ribosome assembly guanine nucleotide exchange factor. UniProt names the product ribosome maturation protein SBDS.
NCBI describes SBDS as highly conserved, and mouse and rat both carry an orthologue named Sbds. Mutations in the gene are associated with the autosomal recessive Shwachman-Bodian-Diamond syndrome. Check the datasheet of any product for its validated reactivity.
Partner proteins of SBDS in 60S ribosomal subunit maturation and translation initiation.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9Y3A5; product specifications from Assay Genie datasheets.