NUDT5
NUDT5 (nudix hydrolase 5, also known as hYSAH1 or YSA1H) is a nuclear Nudix family enzyme. It hydrolyses ADP-sugars and oxidised nucleoside diphosphates, and in the presence of diphosphate it can instead synthesise ATP. Browse Assay Genie's current range of NUDT5 research reagents below, or read on for the biology behind the target.
NUDT5 is a bifunctional enzyme. In the absence of diphosphate it acts as an ADP-sugar pyrophosphatase, hydrolysing modified nucleoside diphosphates with similar activities, including ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP. Other nucleotide sugars are hydrolysed with low activity. In the presence of diphosphate, the enzyme instead mediates synthesis of ATP in the nucleus, converting ADP-ribose to ATP and ribose 5-phosphate.
This nuclear ATP synthesis takes place when NUDT5 is dephosphorylated at Thr-45. The ATP generated is required for extensive, energy-consuming chromatin remodelling events. In vitro, NUDT5 also has decapping activity on 11-mer RNA capped with UDP-GlcNAc, hydrolysing the pyrophosphate bridge of the sugar cap. It does not play a role in U8 snoRNA decapping, although it binds U8 snoRNA.
The NUDT5 gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily; the protein is also listed as ADP-sugar pyrophosphatase. The NCBI Gene summary notes why this activity matters: protein-bound ADP-ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in inhibition of ATP-activated potassium channels. 8-oxoGua, an oxidised form of guanine, can pair with adenine and cytosine in RNA, and its presence in RNA leads to abnormal proteins through translational errors. Mouse and rat orthologues are designated Nudt5.
NUDT5 generates nuclear ATP from ADP-ribose, which is required for energy-consuming chromatin remodelling events.
The enzyme hydrolyses 8-oxo-GDP and 8-oxo-dGDP, making it relevant to studies of oxidised guanine and its effects on genetic information.
NUDT5 hydrolyses ADP-ribose, limiting protein-bound ADP-ribose that can modify amino acid residues and inhibit ATP-activated potassium channels.
In vitro, NUDT5 removes UDP-GlcNAc caps from short RNA, supporting work on non-canonical RNA caps and their processing.
NUDT5 is an ADP-sugar pyrophosphatase that hydrolyses ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP when diphosphate is absent. With diphosphate present, it synthesises ATP in the nucleus from ADP-ribose, supporting chromatin remodelling.
NUDT5 is the HGNC symbol for nudix hydrolase 5. Aliases include hYSAH1 and YSA1H, and the previous name was nudix (nucleoside diphosphate linked moiety X)-type motif 5. UniProt names the protein ADP-sugar pyrophosphatase, EC 3.6.1.13.
Yes. Mouse and rat both have an orthologous gene named Nudt5. Human NUDT5 belongs to the Nudix hydrolase family, a superfamily of enzymes acting on nucleoside diphosphates linked to another moiety X. Check the datasheet of any product for its validated reactivity.
Other Nudix hydrolase family members and nucleotide-metabolism enzymes with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9UKK9; product specifications from Assay Genie datasheets.