RPN1
RPN1 (ribophorin I, also known as OST1) is a subunit of the oligosaccharyltransferase (OST) complex, which carries out the first step of protein N-glycosylation in the endoplasmic reticulum. It is a type I integral membrane protein of the rough endoplasmic reticulum. Browse Assay Genie's current range of RPN1 research reagents below, or read on for the biology behind the target.
RPN1 is a subunit of the oligosaccharyl transferase (OST) complex. This complex catalyses the initial transfer of a defined glycan, Glc(3)Man(9)GlcNAc(2) in eukaryotes, from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. This is the first step in protein N-glycosylation. The process occurs cotranslationally, and the complex associates with the Sec61 complex at the channel-forming translocon that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for maximal enzyme activity.
The RPN1 gene encodes a type I integral membrane protein found only in the rough ER, with the protein also annotated at the melanosome. The protein, formally named dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, belongs to the OST1 family and is classed by HGNC among the oligosaccharyltransferase complex subunits. Orthologous genes are named Rpn1 in mouse and rat.
The NCBI Gene summary describes the encoded protein as part of an N-oligosaccharyl transferase complex that links high-mannose oligosaccharides to asparagine residues in nascent polypeptide chains. The same summary also reports that the protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. Researchers studying ribophorin I, the RPN1 protein or the OST1 alias will find it discussed in the context of glycosylation at the ER membrane.
RPN1 is a subunit of the OST complex that catalyses the first step of N-glycosylation on nascent polypeptide chains.
The OST complex associates with the Sec61 complex at the translocon, linking glycosylation to protein translocation across the endoplasmic reticulum.
RPN1 contributes to transfer of Glc(3)Man(9)GlcNAc(2) from dolichol-pyrophosphate to Asn-X-Ser/Thr motifs, a defined model for studying enzymatic glycan transfer.
NCBI Gene reports RPN1 as part of the 26S proteasome regulatory subunit, possibly mediating binding of ubiquitin-like domains.
RPN1 is a subunit of the oligosaccharyl transferase complex, which transfers a glycan from dolichol-pyrophosphate to asparagine in Asn-X-Ser/Thr motifs of nascent polypeptides. This is the first step of protein N-glycosylation, and it occurs cotranslationally at the ER translocon with the Sec61 complex.
RPN1 is the HGNC symbol for ribophorin I, and OST1 is a listed alias. UniProt names the protein dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, and it belongs to the OST1 family. HGNC places it in the oligosaccharyltransferase complex subunits group.
Yes. Mouse and rat both carry an ortholog named Rpn1. The OST complex catalyses N-glycosylation in eukaryotes, so the pathway is broadly conserved. Check the datasheet of any product for its validated reactivity.
Other oligosaccharyltransferase subunits and ER translocation components with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P04843; product specifications from Assay Genie datasheets.