E2F4
E2F4 (E2F transcription factor 4, also written E2F-4) is a nuclear member of the E2F/DP family that binds DNA with DP partner proteins to regulate genes involved in cell cycle control and DNA replication. It associates with the pocket proteins p107 and p130 and is a subunit of the DREAM complex. Browse Assay Genie's current range of E2F4 research reagents below, or read on for the biology behind the target.
E2F4 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. This motif is found in the promoter region of many genes whose products are involved in cell cycle regulation or DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 (p107) and RBL2 (p130), and in some instances can also bind RB1. Overall, it plays an important role in the suppression of proliferation-associated genes.
E2F4 has a second, specific role in differentiation. It is required for multiciliate cell differentiation, where it works with MCIDAS and E2F5 to bind and activate genes needed for centriole biogenesis.
The E2F4 gene encodes a member of the E2F family, whose members share several evolutionarily conserved domains: a DNA binding domain, a dimerization domain that determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumour suppressor protein association domain embedded within the transactivation domain. The E2F family is central to the control of the cell cycle and to the action of tumour suppressor proteins. It is also a target of the transforming proteins of small DNA tumour viruses. E2F4 binds all three of the tumour suppressor proteins pRB, p107 and p130, with higher affinity for the last two. Mutation of the gene and increased expression may be associated with human cancer. HGNC classes E2F4 with the E2F transcription factors and the DREAM complex subunits, and previously named it E2F transcription factor 4, p107/p130-binding. The mouse ortholog is E2f4.
E2F4 acts with DP proteins in the DRTF1/E2F complex, which controls progression from G1 to S phase.
E2F4 binds pRB, p107 and p130, placing it within the pocket protein pathways that restrain proliferation.
Mutation and increased expression of the E2F4 gene may be associated with human cancer.
E2F4 is required, with MCIDAS and E2F5, to activate genes for centriole biogenesis during multiciliate cell differentiation.
E2F4 is a transcription factor that binds DNA with DP proteins at the E2 recognition site in promoters of cell cycle and DNA replication genes. It binds p107 and p130 with high affinity and helps suppress proliferation-associated genes. It also supports centriole biogenesis in multiciliate cells.
The HGNC name is E2F transcription factor 4, written E2F-4 in some sources. An earlier name was E2F transcription factor 4, p107/p130-binding, which reflects its preferred pocket protein partners. UniProt lists the protein as transcription factor E2F4.
E2F4 belongs to the E2F/DP family and is grouped with the DREAM complex subunits. It works alongside E2F5 and MCIDAS in ciliogenesis. Gene mutation and increased expression may be associated with human cancer. The mouse ortholog is E2f4; check the datasheet of any product for its validated reactivity.
Other E2F family members, pocket proteins and cell cycle regulators related to E2F4 with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q16254; product specifications from Assay Genie datasheets.