TPPP
TPPP (tubulin polymerization promoting protein, also known as p25alpha, TPPP1 or TPPP/p25) is a regulator of microtubule dynamics that promotes myelin sheath elongation. It nucleates, bundles and stabilises microtubules and shows magnesium-dependent GTPase activity. Browse Assay Genie's current range of TPPP research reagents below, or read on for the biology behind the target.
TPPP is a regulator of microtubule dynamics with a key role in myelination, where it promotes elongation of the myelin sheath. In oligodendrocytes it acts as a microtubule nucleation factor, localising to the Golgi apparatus region known as the Golgi outpost and promoting microtubule nucleation, an important step in sheath elongation. It is required for uniform polarised growth of distal microtubules and for directing the branching of proximal processes.
Beyond nucleation, TPPP is involved in microtubule bundling and in stabilising existing microtubules, which maintains the integrity of the microtubule network. It also promotes tubulin acetylation by inhibiting the tubulin deacetylase activity of HDAC6. Phosphorylation by ROCK1 prevents the interaction with HDAC6, which lowers tubulin acetylation and increases cell motility. TPPP additionally shows magnesium-dependent GTPase activity, although the role of this activity is unclear, and it is able to homodimerise.
TPPP also contributes to cell proliferation by regulating the G1/S-phase transition. During early mitosis it is involved in astral microtubule organisation and mitotic spindle orientation, a process regulated by LIMK2 phosphorylation. The protein is found at the Golgi outpost, microtubule organising centre, cytoskeleton, spindle and nucleus. It belongs to the TPPP family, within the HGNC group of tubulin polymerization promoting proteins, and has orthologues named Tppp in mouse and rat. Studies of the TPPP gene and protein, often called p25alpha, centre on cytoskeletal organisation and myelination.
TPPP promotes microtubule nucleation and elongation of the myelin sheath in oligodendrocytes, making it a focus for studies of myelin formation.
TPPP nucleates, bundles and stabilises microtubules, offering a route to examine how the microtubule network is organised and maintained.
By inhibiting HDAC6 deacetylase activity, TPPP links tubulin acetylation to ROCK1 phosphorylation and changes in cell motility.
TPPP regulates the G1/S-phase transition and helps orient the mitotic spindle through astral microtubule organisation, under LIMK2 control.
TPPP regulates microtubule dynamics. In oligodendrocytes it nucleates microtubules at the Golgi outpost to promote myelin sheath elongation, and it also bundles and stabilises microtubules. It promotes tubulin acetylation by inhibiting HDAC6 and has magnesium-dependent GTPase activity of unclear role.
TPPP is also called p25alpha, TPPP1, p25 and TPPP/p25. The HGNC full name is tubulin polymerization promoting protein, and UniProt lists it as tubulin polymerization-promoting protein. It belongs to the TPPP family, and the HGNC group has the same name.
Mouse and rat both have an orthologue named Tppp, so the gene can be studied in common rodent models. TPPP3 is a related member of the same naming group. Check the datasheet of any product for its validated reactivity.
Related members of the tubulin polymerization promoting protein family with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O94811; product specifications from Assay Genie datasheets.