GOPC
GOPC (golgi associated PDZ and coiled-coil motif containing) is a Golgi-associated PDZ domain protein involved in intracellular protein trafficking and degradation. It is also known as PIST, CAL, FIG, GOPC1 and dJ94G16.2. Browse Assay Genie's current range of GOPC research reagents below, or read on for the biology behind the target.
GOPC plays a role in intracellular protein trafficking and degradation. It is reported to regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating the cell surface expression of both channels, and it may also regulate the intracellular trafficking of the ADR1B receptor. GOPC may additionally play a role in autophagy. Together with MARCHF2, it mediates the ubiquitination and lysosomal degradation of CFTR, and overexpression results in CFTR intracellular retention and degradation in lysosomes.
The GOPC gene encodes a Golgi protein with a PDZ domain. PDZ domains are globular and allow proteins that contain them to bind other proteins through short motifs near the C-termini, which is consistent with GOPC acting as an adaptor for membrane protein sorting. HGNC places GOPC in the PDZ domain containing gene group. According to UniProt, the protein is found in the cytoplasm, in the Golgi apparatus membrane and trans-Golgi network membrane, and at the synapse, including the postsynaptic density and dendrites.
Mice deficient in the orthologous protein (Gopc) have globozoospermia and are infertile, linking GOPC to sperm development in this model. Multiple transcript variants encoding different isoforms have been reported for the human gene. The aliases PIST, CAL and FIG appear throughout the literature, so searches for GOPC often need to include these names.
GOPC regulates CFTR surface expression and, with MARCHF2, mediates its ubiquitination and lysosomal degradation, making it relevant to channel trafficking studies.
GOPC may modulate cell surface expression of ASIC3 and influence acid-induced ASIC3 currents, supporting work on channel localisation.
As a Golgi-associated PDZ protein involved in trafficking and degradation, GOPC is a useful subject for studies of intracellular protein sorting.
Gopc-deficient mice show globozoospermia and infertility, so GOPC is studied in the context of sperm development and male fertility.
GOPC plays a role in intracellular protein trafficking and degradation. It may regulate CFTR chloride currents and acid-induced ASIC3 currents by controlling cell surface expression of these channels, and may influence ADR1B receptor trafficking and autophagy. With MARCHF2 it promotes CFTR ubiquitination and lysosomal degradation.
GOPC is also called PIST, CAL, FIG, GOPC1 and dJ94G16.2. The HGNC full name is golgi associated PDZ and coiled-coil motif containing. Multiple transcript variants encoding different isoforms have been described, so check which isoform a reagent targets.
A mouse ortholog, Gopc, exists. Mice deficient in it have globozoospermia and are infertile, showing a role in sperm development. Human GOPC is also studied for its regulation of CFTR trafficking. Check the datasheet of any product for its validated reactivity.
Channels, receptors and ubiquitin ligases linked to GOPC-mediated trafficking and degradation.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9HD26; product specifications from Assay Genie datasheets.