RASSF1
RASSF1 (Ras association domain family member 1; also NORE2A, REH3P21, RDA32 and 123F2) is a potential tumour suppressor with similarity to RAS effector proteins. It supports death receptor-dependent apoptosis and restrains cell cycle progression. Browse Assay Genie's current range of RASSF1 research reagents below, or read on for the biology behind the target.
RASSF1 is a potential tumour suppressor that acts in apoptosis and cell cycle control. It is required for death receptor-dependent apoptosis, where it mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, RASSF1 promotes a conformational change in BAX and its translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation.
RASSF1 also limits proliferation. It negatively regulates progression through the G1/S-phase transition by controlling the accumulation of cyclin D1 protein. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex (APC), inhibiting APC activity and mitotic progression. Isoform A also disrupts interactions among MDM2, DAXX and USP7, which promotes MDM2 self-ubiquitination and contributes to efficient activation of TP53 in cell-cycle checkpoint control after DNA damage. Isoform C does not perform these roles, and no function has been identified for it. The protein is found in the cytoskeleton, including the centrosome and spindle, and in the nucleus. It has also been reported to interact with the DNA repair protein XPA.
The RASSF1 gene encodes a protein similar to RAS effectors, and several alternatively spliced transcript variants encode distinct isoforms. Loss or altered expression of the gene has been associated with the pathogenesis of a variety of cancers, and its inactivation correlates with hypermethylation of its CpG-island promoter region. The previous HGNC name was Ras association (RalGDS/AF-6) domain family member 1, and the gene belongs to the Ras association domain family, which includes RASSF2, RASSF4, RASSF6, RASSF7 and RASSF9.
Inactivation of RASSF1 correlates with hypermethylation of its CpG-island promoter, making it a focus for studies of gene silencing in cancer.
RASSF1 is required for death receptor-dependent apoptosis, acting on STK3/MST2 and STK4/MST1 and, with MOAP1, on BAX translocation.
RASSF1 restrains G1/S progression by controlling cyclin D1 accumulation and inhibits APC activity through CDC20 binding.
Isoform A disrupts MDM2, DAXX and USP7 interactions to promote TP53 activation, and the protein interacts with XPA.
RASSF1 is a potential tumour suppressor. It is required for death receptor-dependent apoptosis, promotes BAX translocation with MOAP1, and inhibits proliferation by limiting cyclin D1 accumulation at the G1/S transition. Isoform A also inhibits APC activity via CDC20 and supports TP53 activation after DNA damage.
RASSF1 is also known as NORE2A, REH3P21, RDA32 and 123F2. Its previous name was Ras association (RalGDS/AF-6) domain family member 1. Several alternatively spliced transcript variants encode distinct isoforms; isoform A is functionally characterised, while no function has been identified for isoform C.
Loss or altered expression of the RASSF1 gene has been associated with a variety of cancers, and its inactivation correlates with promoter CpG-island hypermethylation. The mouse ortholog is Rassf1. Check the datasheet of any product for its validated reactivity.
Other members of the Ras association domain family with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9NS23; product specifications from Assay Genie datasheets.