POT1
POT1 (protection of telomeres 1, also known as hPot1) is a nuclear, single-stranded telomeric DNA-binding protein and a component of the shelterin complex. It contributes to telomere length regulation and the protection of chromosome ends. Browse Assay Genie's current range of POT1 research reagents below, or read on for the biology behind the target.
POT1 is a component of the shelterin complex (telosome), which associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends. Without this protective activity, telomeres are no longer hidden from DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. POT1 binds two or more telomeric single-stranded 5'-TTAGGG-3' repeats (the G-strand), and binds with high specificity to a minimal 5'-TAGGGTTAG-3' sequence, either internally or close to a 3'-end.
POT1 is also a component of the double-stranded telomeric DNA-binding TRF1 complex, which regulates telomere length by cis-inhibition of telomerase. As a single-stranded telomeric DNA-binding protein, it may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance or length to the telomere terminus. It is part of the telomerase ribonucleoprotein complex, which is essential for replicating chromosome termini. Its activity is TERT dependent, but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.
The POT1 gene encodes a nuclear protein of the telombin family. Its roles in telomere maintenance include protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability and abnormal chromosome segregation. Increased transcriptional expression of the gene is associated with stomach carcinogenesis and its progression, and alternatively spliced transcript variants have been described. The earlier HGNC name referred to an S. pombe homolog, and the mouse ortholog is Pot1.
POT1 binds single-stranded telomeric TTAGGG repeats and helps regulate telomere length, making it a central subject in telomere maintenance studies.
POT1 activity is TERT dependent, and the ACD-POT1 heterodimer increases telomerase processivity, which is relevant to studies of telomere elongation.
Shelterin protection hides telomeres from DNA damage surveillance and repair pathways, so POT1 is relevant to chromosome end protection and stability.
Increased POT1 transcription is associated with stomach carcinogenesis and its progression, linking telomere protection to gastric cancer studies.
POT1 binds single-stranded telomeric TTAGGG repeats as part of the shelterin complex. It helps regulate telomere length and protects chromosome ends from being treated as DNA damage. Its activity is TERT dependent, and with ACD it enhances telomerase processivity.
POT1 stands for protection of telomeres 1, and UniProt lists the protein as protection of telomeres protein 1. Aliases include hPot1 and DKFZp586D211. The previous HGNC name was protection of telomeres 1 homolog (S. pombe). Alternatively spliced transcript variants have been described.
Increased POT1 transcription is associated with stomach carcinogenesis and its progression. A mouse ortholog, Pot1, exists. The human protein belongs to the telombin family; check the datasheet of any product for its validated reactivity.
Other targets with products at Assay Genie, including POTE family members that share a similar-looking gene symbol.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9NUX5; product specifications from Assay Genie datasheets.