ADPRH
ADPRH (ADP-ribosylarginine hydrolase, also known as ARH1) is an arginine mono-ADP-ribosylhydrolase that removes mono-ADP-ribose from arginine residues on proteins. It acts in the ADP-ribosylation cycle and belongs to the ADP-ribosylglycohydrolase family. Browse Assay Genie's current range of ADPRH research reagents below, or read on for the biology behind the target.
ADPRH is an enzyme that specifically acts as an arginine mono-ADP-ribosylhydrolase. It mediates the removal of mono-ADP-ribose attached to arginine residues on proteins, reversing this modification. According to NCBI Gene, it catalyses this removal as part of the ADP-ribosylation cycle, a reversible post-translational modification system in which ADP-ribose is attached to and released from protein substrates.
The human ADPRH gene encodes a protein designated ADP-ribosylhydrolase ARH1, and the enzyme is classified under EC 3.2.2.19. It belongs to the ADP-ribosylglycohydrolase family. Orthologues are annotated in mouse and rat, both named Adprh. The RefSeq summary notes that alternatively spliced transcript variants encoding different protein isoforms have been described for the gene.
A noteworthy species difference is recorded in the gene summary: unlike the rat and mouse enzymes, which require DTT for maximal activity, the human enzyme is DTT-independent. This is relevant when designing biochemical assays or comparing activity across species. The official HGNC name is ADP-ribosylarginine hydrolase, and ARH1 remains a widely used alias, so both terms are useful when searching the literature on the ADPRH gene or ARH1 protein.
ADPRH removes mono-ADP-ribose from arginine residues, making it a key enzyme for studying the reversal of protein mono-ADP-ribosylation in the ADP-ribosylation cycle.
The human enzyme is DTT-independent, unlike the rat and mouse enzymes, which is an important consideration when characterising activity and comparing species.
As a specific arginine mono-ADP-ribosylhydrolase, ADPRH helps define how arginine-linked modifications on proteins are reversed.
Alternatively spliced transcript variants encoding different isoforms have been described, supporting work on ADPRH gene regulation and protein variants.
ADPRH specifically acts as an arginine mono-ADP-ribosylhydrolase. It removes mono-ADP-ribose attached to arginine residues on proteins, catalysing this step within the ADP-ribosylation cycle. It is classified as EC 3.2.2.19 and belongs to the ADP-ribosylglycohydrolase family.
ADPRH is the HGNC symbol for ADP-ribosylarginine hydrolase, and ARH1 is a recognised alias. UniProt names the protein ADP-ribosylhydrolase ARH1. NCBI Gene reports that alternatively spliced transcript variants encoding different protein isoforms have been described.
Mouse and rat both carry an Adprh orthologue. According to the NCBI Gene summary, the rat and mouse enzymes need DTT for maximal activity, whereas the human enzyme is DTT-independent. Check the datasheet of any product for its validated reactivity.
Other ADP-ribosylation enzymes and post-translational modification targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P54922; product specifications from Assay Genie datasheets.