CDC25B
CDC25B (cell division cycle 25B, also known as MPIP2 or M-phase inducer phosphatase 2) is a tyrosine protein phosphatase that drives cells into mitosis. It acts as a dosage-dependent inducer of mitotic progression by dephosphorylating and activating CDK1. Browse Assay Genie's current range of CDC25B research reagents below, or read on for the biology behind the target.
CDC25B is a tyrosine protein phosphatase that functions as a dosage-dependent inducer of mitotic progression. It directly dephosphorylates CDK1 and stimulates its kinase activity; the NCBI Gene summary describes this as removing two phosphate groups from the cyclin dependent kinase CDC2 (CDK1), which is required for entry into mitosis. CDC25B is needed for G2/M progression of the cell cycle and for abscission during cytokinesis, where it acts in an ECT2-dependent manner.
The CDC25B protein belongs to the MPI phosphatase family and is classed by HGNC among the Class III Cys-based CDC25 phosphatases. It is a member of the CDC25 family, alongside CDC25A and CDC25C. In UniProt it is annotated at the centrosome and the spindle pole. Its localisation changes through the cell cycle: CDC25B shuttles between the nucleus and the cytoplasm using nuclear localisation and nuclear export signals, and is nuclear in M and G1 phases before moving to the cytoplasm during S and G2.
The CDC25B gene produces multiple transcript variants, and UniProt notes that the three isoforms seem to differ in their level of activity. CDC25B is reported to have oncogenic properties, although its role in tumour formation has not been determined. The mouse and rat orthologues are both named Cdc25b. Researchers studying cell cycle control often examine CDC25B alongside CDK1 and the other CDC25 phosphatases.
CDC25B activates CDK1 by dephosphorylation and is required for G2/M progression, making it central to studies of how cells commit to mitosis.
CDC25B is required for abscission during cytokinesis in an ECT2-dependent manner, linking the phosphatase to the final stage of cell division.
CDC25B has reported oncogenic properties, although its role in tumour formation has not been determined, so it remains a subject of cancer research.
CDC25B moves between nucleus and cytoplasm through localisation and export signals, with its location shifting across the phases of the cell cycle.
CDC25B is a tyrosine protein phosphatase that induces mitotic progression in a dosage-dependent manner. It dephosphorylates CDK1 and stimulates its kinase activity, and it is required for G2/M progression and for abscission during cytokinesis in an ECT2-dependent manner.
CDC25B is also called MPIP2 and M-phase inducer phosphatase 2, and HGNC lists its full name as cell division cycle 25B. The gene has multiple transcript variants, and UniProt reports three isoforms that seem to differ in their level of activity.
CDC25B is reported to have oncogenic properties, although its role in tumour formation has not been determined. Orthologues exist in mouse and rat, both named Cdc25b. Check the datasheet of any product for its validated reactivity.
Other CDC25 phosphatases, cell division cycle proteins and mitotic regulators with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P30305; product specifications from Assay Genie datasheets.