TTF2
TTF2 (transcription termination factor 2, also known as HuF2 or ZGRF6) is a dsDNA-dependent ATPase of the SNF2/RAD54 helicase family. It terminates transcription by removing RNA polymerase II from the DNA template, and it has also been linked to pre-mRNA splicing. Browse Assay Genie's current range of TTF2 research reagents below, or read on for the biology behind the target.
TTF2 is a double-stranded DNA-dependent ATPase that acts as a transcription termination factor. It couples ATP hydrolysis to the removal of RNA polymerase II from the DNA template, which brings transcription to an end. It may also contribute to the repression of transcription during mitosis, and it may be involved in pre-mRNA splicing.
The TTF2 gene encodes a member of the SWI2/SNF2 family of proteins, which have a critical role in altering protein-DNA interactions. UniProt places the protein in the SNF2/RAD54 helicase family. It has been shown to have both dsDNA-dependent ATPase activity and RNA polymerase II termination activity. UniProt records the protein in the cytoplasm and the nucleus. The mouse ortholog is Ttf2.
The splicing role is supported by interaction data. The TTF2 protein interacts with cell division cycle 5-like (CDC5L) and associates with human splicing complexes. This places it at the point where transcription termination and RNA processing meet. HGNC classifies the gene within the GRF-type zinc fingers, the SNF2 related family and the microRNA protein coding host genes. Its previous name was transcription termination factor, RNA polymerase II, and the aliases HuF2 and ZGRF6 are also in use. Researchers studying the TTF2 gene or protein may find these names in older literature.
TTF2 removes RNA polymerase II from the DNA template in an ATP-dependent step, making it a model for studying how transcription ends.
TTF2 may contribute to the repression of transcription during mitosis, which makes it relevant to studies of gene expression through cell division.
TTF2 associates with human splicing complexes and interacts with CDC5L, linking it to pre-mRNA processing.
As a SWI2/SNF2 family member, TTF2 helps researchers examine how ATP-driven enzymes alter protein-DNA interactions.
TTF2 is a dsDNA-dependent ATPase that works as a transcription termination factor. It couples ATP hydrolysis with the removal of RNA polymerase II from the DNA template. It may also contribute to mitotic transcription repression and may be involved in pre-mRNA splicing.
TTF2 is also called HuF2 and ZGRF6. HGNC lists the full name as transcription termination factor 2, and the previous name as transcription termination factor, RNA polymerase II. The protein is classed as an EC 3.6.4.- enzyme.
TTF2 belongs to the SNF2/RAD54 helicase family, also described as the SWI2/SNF2 family. These proteins alter protein-DNA interactions. A mouse ortholog, Ttf2, exists; check the datasheet of any product for its validated reactivity.
Other SNF2 family ATPases, splicing-complex partners and RNA polymerase II components with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9UNY4; product specifications from Assay Genie datasheets.