The TTF2 Antibody (CAB7751) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.TTF2 is involved in the termination of transcription by RNA polymerase II and has been implicated in various cellular processes, including RNA processing and DNA repair. By targeting TTF2, researchers can investigate its function in different cell types and gain insights into its role in transcriptional regulation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TTF2 Antibody
SKU:
CAB7751
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
F2, HuF2, ZGRF6, TTF2
Positive Sample:
MCF7, HL-60, SKOV3, Jurkat
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
130kDa
Observed MW:
130kDa
This gene encodes a member of the SWI2/SNF2 family of proteins, which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.
Purification Method
Affinity purification
Gene ID
8458
RRID
AB_2772749
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TTF2 Rabbit pAb (CAB7751) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Immunofluorescence analysis of A549 cells using TTF2 Rabbit pAb (CAB7751). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
