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Anti-TTF2 Antibody (CAB7751)

SKU:
CAB7751
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-TTF2 Antibody CAB7751
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-TTF2 Antibody CAB7751
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Description

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Antibody Name:Anti-TTF2 Antibody
Antibody SKU:CAB7751
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TTF2 (NP_003585.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:MCF7, HL-60, SKOV3, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TTF2 (NP_003585.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEEV RCPE HGTF CFLK TGVR DGPN KGKS FYVC RADT CSFV RATD IPVS HCLL HEDF VVEL QGLL LPQD KKEY RLFF RCIR SKAE GKRW CGSI PWQD PDSK EHSV SNKS QHAS ETFH HSSN WLRN PFKV LDKN QEPA LWKQ LIKG EGEE KKAD KKQR EKGD QLFD QKKE QKPE MMEK DLSS GLVP KKKQ SVVQ EKKQ EEGA EIQC EAET GGTH KRDF SEIK SQQC QGNE LTRP SASS QEKS SGKS QDVQ RESE PLRE KVTQ LLPQ NVHS HN
Gene ID:8458
Uniprot:Q9UNY4
Cellular Location:Cytoplasm, Nucleus
Calculated MW:75kDa/129kDa
Observed MW:130kDa
Synonyms:TTF2, HuF2, ZGRF6
Background:This gene encodes a member of the SWI2/SNF2 family of proteins, which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.
UniProt Protein Function:TTF2: DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Helicase; EC 3.6.4.-; Transcription regulation; EC 3.6.1.-; RNA splicing; DNA-binding

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: spliceosome; transcription elongation factor complex; cytoplasm

Molecular Function:DNA-dependent ATPase activity; protein binding; DNA binding; zinc ion binding; ATP-dependent helicase activity; ATP binding

Biological Process: regulation of transcription, DNA-dependent; RNA splicing; mRNA processing; termination of RNA polymerase II transcription; transcription termination

NCBI Summary:This gene encodes a member of the SWI2/SNF2 family of proteins, which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UNY4
NCBI GenInfo Identifier:119577071
NCBI Gene ID:8458
NCBI Accession:EAW56667.1
UniProt Related Accession:Q9UNY4
Molecular Weight:74kDa
NCBI Full Name:transcription termination factor, RNA polymerase II, isoform CRA_a
NCBI Synonym Full Names:transcription termination factor 2
NCBI Official Symbol:TTF2  
NCBI Official Synonym Symbols:HuF2; ZGRF6  
NCBI Protein Information:transcription termination factor 2
UniProt Protein Name:Transcription termination factor 2
UniProt Synonym Protein Names:Lodestar homolog; RNA polymerase II termination factor; Transcription release factor 2
Protein Family:Transcription termination factor
UniProt Gene Name:TTF2  
UniProt Entry Name:TTF2_HUMAN
Anti-TTF2 Antibody (CAB7751)Western blot analysis of extracts of various cell lines, using TTF2 antibody (CAB7751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-TTF2 Antibody (CAB7751)Immunofluorescence analysis of A549 cells using TTF2 antibody (CAB7751). Blue: DAPI for nuclear staining.

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