PLCB1
PLCB1 (phospholipase C beta 1) is a phosphoinositide-specific phospholipase that converts phosphatidylinositol 4,5-bisphosphate into the second messengers diacylglycerol and inositol 1,4,5-trisphosphate. It is also known as KIAA0581, PLC-I and PLC154, and it acts downstream of G protein-coupled receptors. Browse Assay Genie's current range of PLCB1 research reagents below, or read on for the biology behind the target.
PLCB1 catalyses the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). The reaction uses calcium as a cofactor. Because DAG and IP3 are central second messengers, the enzyme plays an important role in the intracellular transduction of many extracellular signals. UniProt describes it as mediating intracellular signalling downstream of G protein-coupled receptors, and it is also reported to regulate the function of the endothelial barrier.
The PLCB1 gene is activated by two G-protein alpha subunits, alpha-q and alpha-11, which links receptor stimulation to phosphoinositide turnover. The encoded protein is recorded in UniProt as localised to the nuclear membrane and the cytoplasm. NCBI Gene reports two transcript variants that encode different isoforms. Researchers studying the PLCB1 protein often examine it alongside other beta-class enzymes such as PLCB3.
HGNC classifies the gene within the phospholipases group, specifically the C2 domain containing phospholipases. Its earlier approved name was phospholipase C, beta 1 (phosphoinositide-specific), and the aliases KIAA0581, PLC-I and PLC154 still appear in the literature and in older database records. The enzyme has mouse and rat orthologs, both named Plcb1, which allows its signalling role to be studied in common model systems.
PLCB1 mediates intracellular signalling downstream of G protein-coupled receptors, making it a key readout for Gq- and G11-linked pathways.
The enzyme generates DAG and IP3 from phosphatidylinositol 4,5-bisphosphate, so it is central to studies of phosphoinositide turnover.
PLCB1 regulates the function of the endothelial barrier, which is relevant to work on vascular permeability and integrity.
As a C2 domain containing phospholipase, PLCB1 is used for comparing isoform-specific roles within the phospholipase C beta group.
PLCB1 hydrolyses 1-phosphatidylinositol 4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate, using calcium as a cofactor. In this way it transmits signals from G protein-coupled receptors into the cell. UniProt also notes a role in regulating endothelial barrier function.
PLCB1 is also called KIAA0581, PLC-I and PLC154, and was previously named phospholipase C, beta 1 (phosphoinositide-specific). The UniProt protein name is 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1. NCBI Gene reports two transcript variants encoding different isoforms.
Yes. Both mouse and rat have an ortholog named Plcb1, so the enzyme can be studied in standard rodent models. Check the datasheet of any product for its validated reactivity before choosing a species.
Other phospholipase C beta family members and signalling enzymes with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9NQ66; product specifications from Assay Genie datasheets.