PYM1
PYM1 (PYM1 exon junction complex associated factor, also known as PYM or WIBG) is a key regulator of the exon junction complex. It disassembles the complex from spliced mRNAs in a translation-dependent manner, allowing the components to be recycled. Browse Assay Genie's current range of PYM1 research reagents below, or read on for the biology behind the target.
The exon junction complex (EJC) is a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs. It acts as a positional landmark for the intron-exon structure of genes and directs post-transcriptional processes in the cytoplasm, including mRNA export, nonsense-mediated mRNA decay (NMD) and translation. PYM1 is a key regulator of this complex. It acts as an EJC disassembly factor, disrupting the mature EJC on spliced mRNAs so that the complex can be removed and recycled once translation has occurred.
PYM1 associates with the 40S ribosomal subunit, and NCBI Gene annotates ribosome binding as one of its activities. This association probably prevents translation-independent disassembly of the EJC, by restricting PYM1 activity to mRNAs that have been translated. PYM1 interferes with NMD and enhances translation of spliced mRNAs, probably by antagonising EJC functions. It may also bind RNA, although the relevance of this in vivo remains unclear: one study detected RNA binding, while another found no RNA-binding activity independent of the EJC.
The protein is annotated in the cytoplasm and in the nucleus, within the nucleolus and nucleoplasm, and belongs to the pym family. Its UniProt name is Partner of Y14 and mago. The older HGNC name was within bgcn homolog (Drosophila), which explains the alias WIBG, and the gene was also called PYM homolog 1, exon junction complex associated factor. The mouse ortholog is Pym1. Researchers studying the PYM1 gene or PYM1 protein usually do so in the context of EJC recycling, NMD and translational control.
PYM1 disassembles the EJC from spliced mRNAs, making it a direct tool for studying how the complex is removed and recycled.
PYM1 interferes with NMD, probably by antagonising EJC functions, so it is relevant to studies of mRNA surveillance.
PYM1 enhances translation of spliced mRNAs and binds the 40S ribosomal subunit, linking EJC turnover to the translation machinery.
Whether PYM1 binds RNA independently of the EJC remains unresolved, leaving open questions on its mechanism in vivo.
PYM1 is a key regulator of the exon junction complex. It acts as an EJC disassembly factor, removing the complex from spliced mRNAs in a translation-dependent way so it can be recycled. It also interferes with nonsense-mediated mRNA decay and enhances translation of spliced mRNAs.
PYM1 is also known as PYM and WIBG. The UniProt protein name is Partner of Y14 and mago. Earlier HGNC names were PYM homolog 1, exon junction complex associated factor, and within bgcn homolog (Drosophila). The current approved name is PYM1 exon junction complex associated factor.
The mouse has an ortholog named Pym1, and the human protein belongs to the pym family. Human PYM1 is annotated in the cytoplasm, nucleolus and nucleoplasm. Check the datasheet of any product for its validated reactivity.
Exon junction complex components, nonsense-mediated decay factors and translation regulators studied alongside PYM1.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9BRP8; product specifications from Assay Genie datasheets.